[A new phenolic glycoside from roots of Aconitum pendulum]

Ten compounds were isolated from the 95% ethanol extract of the roots of Aconitum pendulum using silica gel chromatography, gel chromatography, and semi-preparative high-performance liquid chromatography. Their structures were identified by various spectroscopic methods, including UV, IR, HR-ESI-MS, and NMR. The compounds were identified as two phenolic compounds: 4-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyloxy]-3-methoxypropiophenone(1) and corchoionoside C(2); two lignans:(+)-oxabicyclooctalignan(3) and(-)-oxabicyclooctalignan(4); five diterpenoid alkaloids: benzoylaconine(5), 14-benzoyl-8-O-methylaconine(6),(-)-(A-b)-14α-benzoyloxy-N-ethyl-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxyaconitane(7), smirnotine A(8), and aconitine(9); and one coumarin compound: scopoletin(10). Among them, compound 1 is a novel phenolic glycoside, while compounds 3, 4, 7, 8, and 10 were isolated from A. pendulum for the first time. The anti-inflammatory activities of the compounds were evaluated using an LPS-induced RAW264.7 macrophage inflammation model. Compounds 5, 6, and 10 inhibited LPS-induced nitric oxide(NO) production in the cells, with IC_(50) values of(34.1±1.3),(28.0±1.0), and(20.3±1.7) μmol·L~(-1), respectively.