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Updated: May 1, 2026

Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
Published on: July 10, 2012
[A new lignan from Syringa pinnatifolia and its cardioprotective activity in vitro]
Qiu-Yuan Ding1, Yi-Ze Xiao1, Jing-Jing Sun1
1Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 102488, China.
Abstract:
This study aims to investigate the chemical constituents of Syringa pinnatifolia. The lignan subfraction I_3 was isolated and purified by the ODS column chromatography and semi-preparative HPLC and structurally elucidated by ~1H-NMR, ~(13)C-NMR, 2D NMR, MS, and ECD data analysis. The protective effect was evaluated based on the model of oxygen-glucose deprivation(OGD)-induced injury in HL-1 cardiomyocytes. Two lignans were isolated and identified as(-)-alashinol A(1) and alashinol A(2), of which 1 was a new lignan and exhibited a significant protective effect against the OGD-induced injury in HL-1 cardiomyocytes. A new lignan with cardioprotective activity in vitro was described in the stem of S. pinnatifolia, which provided a reference for clarifying the pharmacological substances of this herbal medicine.
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