[Chemical constituents of alkaloids from roots of Berberis polyantha]

The chemical constituents of the roots of Berberis polyantha were investigated by various chromatographic separation methods, including silica gel column chromatography, SP825 column chromatography, polyamide column chromatography, reversed-phase C_(18) column chromatography, and preparative high-performance liquid chromatography. The structures of compounds were identified through physicochemical properties and spectroscopic techniques(1D NMR, 2D NMR, and X-ray). From the methanol extract of the roots of B. polyantha, six compounds were isolated and identified as polyanthaine(1), corydaldine(2), noroxyhydrastinine(3), 8-oxypalmatine(4), 8-oxyberberine(5), and rugosinone(6). Compound 1 was a novel compound and named polyanthaine, and other compounds were isolated from this plant for the first time. The anti-inflammatory effect of the compounds was evaluated by measuring the release of nitric oxide(NO) in the culture of lipopolysaccharide(LPS)-induced RAW264.7 macrophages. At a concentration of 10 μmol·L~(-1), compound 1-3,5,6 demonstrated inhibitory activity on the release of NO in LPS-induced RAW264.7 cells.