[Chemical constituents from Sageretia gracilis]

The water extract of the stem of Sageretia gracilis was separated and purified using Sephadex LH-20, RP-C_(18), silica gel column chromatography, and semi-preparative high performance liquid chromatography(HPLC). Based on physicochemical properties, modern spectroscopic techniques, and comparison with literature, 12 compounds were identified as methyl 4-hydroxylbenzoate 1-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(1), 6-hydroxy-2,4-dimethoxyphenyl-1-O-β-D-furanopyranosyl-(1→6)-β-D-glucopyranosyl-4-hydroxybenzoic acid methyl ester(2), tachioside(3), 2,4,6-trimethoxyphenol-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyronoside(4), methyl syringate-α-L-rhamnoside(5),(7R,8R)-Δ~(7')-3,3'-dimethoxy-4,7,9,9'-tetrahydroxy-8-O-4'-neolignan-7-O-β-D-glucopyranoside(6),(7S,8R)-dihydrodehydrodiconiferyl alcohol-4-O-β-D-glucopyranoside(7), 2,6-dimethoxy-4-(prop-2-enyl)phenyl-O-β-D-glu-(1→6)-β-D-glucoside(8), 5-hydroxy-2-[2-(4-hydroxyphenyl) acetyl]-3-methoxylbenzoic acid(9), icariside E_3(10), 5-(β-D-glucopyranosyloxy)-2-hydroxy benzoic methyl ester(11), and seco-dehydrodiconiferyl alcohol-4-O-β-D-glucopyranoside(12). Among them, compounds 1 and 2 are new compounds, named sagergracioside A and sagergracioside B, respectively. All compounds were obtained from S. gracilis for the first time.