Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

506
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
506
Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

1.6K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
1.6K
Bacterial Signaling01:30

Bacterial Signaling

40.0K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
40.0K
Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

1.5K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
1.5K
Bacterial Growth Curve01:28

Bacterial Growth Curve

2.1K
The bacterial growth curve is a fundamental concept in microbiology that describes the dynamics of bacterial population growth in a closed system with controlled environmental conditions, such as temperature and nutrient availability. This curve is divided into four distinct phases: lag, log (exponential), stationary, and death phases, each reflecting a unique stage of bacterial adaptation and growth. During the lag phase, bacteria acclimate to their surroundings by synthesizing essential...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phage-based microbiome manipulation reveals ecological interactions within gut communities.

bioRxiv : the preprint server for biology·2026
Same author

Repression of bacterial gene expression by antivitamin B<sub>12</sub> binding to a cobalamin riboswitch.

RSC chemical biology·2026
Same author

Cobamide-based interactions between soil bacteria can be predicted based on monoculture growth.

bioRxiv : the preprint server for biology·2025
Same author

The Corrinoid Model for Dissecting Microbial Community Interactions Across Scales.

Annual review of microbiology·2025
Same author

Microbes display broad diversity in cobamide preferences.

mSystems·2025
Same author

Laboratory evolution of <i>E. coli</i> with a natural vitamin B<sub>12</sub> analog reveals roles for cobamide uptake and adenosylation in methionine synthase-dependent growth.

Journal of bacteriology·2025
Same journal

Whole-Embryo 3D Quantification Reveals Conserved Topological Design and Scaling of Germ Layers in Xenopus.

bioRxiv : the preprint server for biology·2026
Same journal

scRNA-seq and genomics analyses reveal key mechanisms of inverted papilloma-associated sinonasal squamous cell carcinoma malignant transformation.

bioRxiv : the preprint server for biology·2026
Same journal

M1C IS NECESSARY FOR DARAXONRASIB RESISTANCE OF NSCLC KRAS(G12C) MUTANT CELLS.

bioRxiv : the preprint server for biology·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jan 13, 2026

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

10.6K

Quenching corrinoid-based interactions in a model bacterial coculture.

Zachary F Hallberg1, Zoila I Alvarez-Aponte1, Alison C Gaudinier1

  • 1Department of Plant & Microbial Biology, University of California, Berkeley, Berkeley, CA, 94720 USA.

Biorxiv : the Preprint Server for Biology
|January 9, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a metabolite quenching strategy to disrupt microbial metabolic interactions. This method uses a high-affinity binding protein to block nutrient sharing, enabling precise microbiome manipulation and community analysis.

Keywords:
corrinoidscross-feedingmicrobial ecologynutritional immunityvitamin B12

More Related Videos

Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

14.0K
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.6K

Related Experiment Videos

Last Updated: Jan 13, 2026

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
07:00

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Published on: October 15, 2019

10.6K
Using Coculture to Detect Chemically Mediated Interspecies Interactions
08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

14.0K
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.6K

Area of Science:

  • Microbiology
  • Metabolic Engineering
  • Systems Biology

Background:

  • Microbial community structure is influenced by metabolic interdependencies.
  • Controlling metabolite availability is crucial for understanding and manipulating microbial communities.
  • Corrinoids (vitamin B12 family) are essential cofactors involved in microbial metabolism and cross-feeding.

Purpose of the Study:

  • To develop a novel strategy for disrupting specific metabolic interactions within microbial communities.
  • To investigate the role of corrinoid cross-feeding in microbial community dynamics.
  • To establish a tool for quantifying metabolite release rates and enabling precision microbiome manipulation.

Main Methods:

  • Developed a metabolite 'quenching' strategy using a high-affinity corrinoid-binding protein (BtuG).
  • Utilized a model coculture of *Sinorhizobium meliloti* (corrinoid producer) and engineered *Escherichia coli* (corrinoid-dependent).
  • Demonstrated corrinoid sequestration and blockage of corrinoid-dependent growth.

Main Results:

  • Successfully demonstrated corrinoid quenching by BtuG, disrupting metabolic cross-feeding.
  • Quantified cobalamin release rate from *S. meliloti*, showing dependence on growth phase.
  • Observed a maximum release rate of approximately 40 cobalamin molecules per minute per cell in late exponential phase.

Conclusions:

  • Established a metabolite quenching strategy to selectively block microbial interactions.
  • This approach can be broadly applied to dissect microbial community structure and function.
  • High-affinity 'molecular sponges' can identify key nutrients and empower precision microbiome manipulation.