Mean Platelet Volume in Blood vs. Platelet-Rich Plasma: Demographic Analysis and Intrasubject Variability in PRP...

Area of Science:

• Orthopedics
• Hematology
• Biotechnology

Background:

• Platelet-rich plasma (PRP) is utilized for musculoskeletal conditions.
• Mean platelet volume (MPV) is a marker of platelet activation and function.
• Understanding MPV variations in PRP is crucial for therapeutic efficacy.

Purpose of the Study:

• To compare inter- and intraindividual differences in MPV between whole blood and PRP.
• To assess the influence of demographic factors (sex, age, BMI) on MPV in PRP.
• To evaluate the variability of MPV within and between patients receiving PRP treatments.

Main Methods:

• Retrospective analysis of 877 patients undergoing PRP therapy for musculoskeletal conditions.
• Comparison of MPV values in whole blood versus PRP samples.
• Analysis of intrasubject MPV variability in a subgroup of 298 patients with multiple PRP doses.

Main Results:

• MPV was significantly lower in PRP compared to whole blood (p < 0.001).
• Age, sex, and BMI did not significantly affect MPV.
• PRP exhibited greater intersubject variability in MPV than whole blood, despite low intrasubject variability.

Conclusions:

• The centrifugation process significantly reduces MPV, altering platelet characteristics in PRP.
• Demographic factors do not influence platelet size, but centrifugation protocols are key to PRP composition.
• Standardization of PRP preparation protocols is essential for consistent biological quality and therapeutic outcomes in musculoskeletal treatments.