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Researchers developed novel lanthanide complexes for multimodal imaging. A new DOTA-type ligand enables stable gadolinium (Gd3+) MRI contrast agents and europium (Eu3+) optical probes with excellent water solubility and targeting capabilities.

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Area of Science:

  • Coordination Chemistry
  • Biomedical Imaging
  • Materials Science

Background:

  • Lanthanide (Ln3+) complexes offer unique magnetic and optical properties for multimodal imaging probes.
  • Developing single ligands that meet requirements for both MRI and optical probes is challenging.

Purpose of the Study:

  • To design and synthesize a novel DOTA-type ligand for creating stable, multimodal lanthanide complexes.
  • To evaluate the potential of these complexes as MRI contrast agents and optical imaging probes.

Main Methods:

  • Synthesis of a DOTA-type ligand with a π-extended acetophenone moiety.
  • Formation and characterization of lanthanide (Ln3+) complexes.
  • Assessment of kinetic inertness and thermodynamic stability.
  • Evaluation of *in vivo* MRI contrast properties of the Gd3+ complex.
  • Evaluation of *in vitro* and *in vivo* optical imaging properties of the Eu3+ complex using one-photon and two-photon (2P) excitation.

Main Results:

  • The synthesized ligand successfully formed kinetically inert and thermodynamically stable Ln3+ complexes.
  • The Gd3+ complex demonstrated positive *in vivo* MRI characteristics with good contrast and rapid renal clearance.
  • The Eu3+ complex exhibited excellent one-photon and two-photon absorption, enabling high-quality *in vivo* 2P microscopy imaging in zebrafish embryos and *in vitro* imaging of living cells.

Conclusions:

  • A versatile DOTA-type ligand was developed for multimodal lanthanide-based imaging agents.
  • The resulting Gd3+ and Eu3+ complexes show significant promise for advanced *in vivo* MRI and optical imaging applications.