Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

16.6K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
16.6K
Stem Cell Niche01:26

Stem Cell Niche

6.2K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
6.2K
Cellular Differentiation00:57

Cellular Differentiation

5.0K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.0K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.7K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
Mitochondria01:37

Mitochondria

19.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
19.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electron transport chain complex I and mitochondrial fusion regulate ROS for differentiation in Drosophila neural stem cells.

Stem cell reports·2026
Same author

MIM triggers formin to Arp2/3-based actin assembly in membrane remodeling in Drosophila embryos.

The Journal of cell biology·2026
Same author

Intelligently Actuating Dual-Barrier Hyaluronic Acid-Functionalized Inflammation-Responsive Nanohydrogel for Targeted Rheumatoid Arthritis Therapy.

ACS applied materials & interfaces·2025
Same author

Targeted Nasal Route Delivery of Cationic Anti-TB Drug-Loaded Nano-embedded Microparticles for Mycobacterial Elimination in the CNS.

Molecular pharmaceutics·2025
Same author

Association of visual impairment with malnutrition among elderly Indians: A pilot study.

Bioinformation·2025
Same author

Pharmacokinetic and Pharmacodynamics of Clofazimine Nano-in-Microparticles: Enhanced Brain Delivery and CNS Tuberculosis Amelioration via Intranasal Administration.

ACS infectious diseases·2025

Related Experiment Video

Updated: Jan 13, 2026

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
12:42

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells

Published on: June 17, 2008

14.7K

Mitochondrial dynamics and signaling in stem cell differentiation.

Rahul Kumar Verma1, Somya Madan1, Richa Rikhy1

  • 1Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pune 411008, India.

Journal of Cell Science
|January 9, 2026
PubMed
Summary

Mitochondrial dynamics, involving fusion and fission, are crucial for stem cell renewal and differentiation. Their interplay with signaling pathways regulates cell fate and impacts various biological systems.

Keywords:
Drp1MarfMfnMitochondriaOpa1SignalingStem cells

More Related Videos

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
07:57

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Published on: December 26, 2019

12.9K
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

7.3K

Related Experiment Videos

Last Updated: Jan 13, 2026

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
12:42

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells

Published on: June 17, 2008

14.7K
Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
07:57

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Published on: December 26, 2019

12.9K
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

7.3K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Mitochondrial dynamics, regulated by fusion and fission, are essential for cellular functions like energy production and apoptosis.
  • These dynamics influence development, growth, differentiation, and disease processes.
  • Key proteins like Drp1, Fis1, Opa1, Mfn1, and Mfn2 control mitochondrial morphology.

Purpose of the Study:

  • To explore the interplay between mitochondrial fusion/fission proteins and critical signaling pathways.
  • To understand how mitochondrial morphology and activity regulate stem cell maintenance and differentiation.
  • To examine the impact of mitochondrial dynamics on stem cell division across diverse systems.

Main Methods:

  • Review of existing literature on mitochondrial dynamics and signaling pathways.
  • Analysis of the roles of proteins regulating mitochondrial fission (Drp1, Fis1) and fusion (Opa1, Mfn1, Mfn2).
  • Investigation of signaling cascades including Notch, receptor tyrosine kinase, JNK, Hippo, and mTOR.

Main Results:

  • Mitochondrial morphology and activity are regulated by signaling pathways crucial for stem cell maintenance.
  • Stem cell renewal and differentiation states are dependent on the regulation of signaling pathways by mitochondrial dynamics.
  • Mitochondrial dynamics play a critical role in regulating stem cell division for renewal and differentiation.

Conclusions:

  • Mitochondrial morphology and activity are central regulators of stem cell division, impacting renewal and differentiation.
  • The interplay between mitochondrial dynamics and signaling pathways is vital for controlling cell fate.
  • Understanding these mechanisms offers insights into development and disease.