Related Experiment Video

Updated: Jan 13, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

17.3K

MAPK-related mRNA and miRNA Expression in LPS-treated Keratinocytes: Implications for Psoriasis Inflammation

Aleksandra Plata-Babula1, Michał Wójcik2, Amelia Głowaczewska3

  • 1Department of Nursing and Maternity, High School of Strategic Planning in Dabrowa Gornicza, Dabrowa Gornicza, Poland.

Current Pharmaceutical Biotechnology
|January 9, 2026
PubMed
Abstract

Insights

Lipopolysaccharide (LPS) stimulation in skin cells alters key inflammation pathways and their regulators. This study identifies specific microRNAs (miRNAs) and messenger RNAs (mRNAs) involved in psoriasis, offering potential therapeutic targets.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Immunology

Background:

  • Psoriasis is a chronic autoimmune skin disease driven by immune dysregulation and keratinocyte overgrowth.
  • The mitogen-activated protein kinase (MAPK) signaling pathway is crucial in psoriatic skin inflammation.

Purpose of the Study:

  • To investigate the expression of MAPK-related mRNAs and their regulatory miRNAs in lipopolysaccharide (LPS)-stimulated keratinocytes.
  • To identify potential molecular markers and therapeutic targets for psoriasis.

Main Methods:

  • Oligonucleotide microarrays were used to analyze differential gene and miRNA expression in HaCaT cells post-LPS exposure.
  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) validated mRNA and protein level changes.

Main Results:

  • Significant differential expression was observed in 28 out of 248 MAPK-associated mRNAs, with some downregulated (e.g., DUSP1, MAP2K2) and others upregulated (e.g., TGFB1, IL1B).
  • Protein expression changes corroborated the mRNA findings.
  • Four miRNAs (miR-34a, miR-4692a, miR-200-5p, miR-1275) showed inverse expression patterns compared to their predicted mRNA targets.

Conclusions:

  • LPS-induced inflammation in keratinocytes leads to coordinated dysregulation of MAPK signaling components and regulatory miRNAs.
  • The identified miRNA-mRNA interactions provide insights into psoriasis pathophysiology and suggest potential biomarkers or therapeutic strategies for chronic skin inflammation.
Keywords:
MAPK signaling pathwaysPsoriasisdualspecificity phosphatases (DUSPs).keratinocyte cell linelinelipopolysaccharidemicroarray analysis

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