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Myoglobin Affects Tissue-Specific Transcriptome, Heart Regeneration and Whole Animal Metabolic Rates.

Rasmus Hejlesen1,2, Ciska Bakkeren1, Christian Damsgaard1

  • 1Department of Biology, Aarhus University, Aarhus, Denmark.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
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Summary
This summary is machine-generated.

Loss of myoglobin (Mb) in zebrafish enhances swimming speed and hypoxia tolerance. This study reveals Mb’s role in regulating metabolic rate and cardiac regeneration.

Keywords:
heartmetabolic ratemyoglobinrespirometryskeletal muscleswimming performancetranscriptomicszebrafish

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Area of Science:

  • Physiology
  • Molecular Biology
  • Genetics

Background:

  • Myoglobin (Mb) is a muscle protein with debated in vivo functions.
  • Previous studies in knockout mice yielded conflicting results on Mb's physiological roles.

Purpose of the Study:

  • Investigate the in vivo consequences of myoglobin loss using a zebrafish model.
  • Analyze effects on skeletal muscle, transcriptomics, and metabolic rates.

Main Methods:

  • Generated a CRISPR-Cas zebrafish myoglobin knockout model.
  • Assessed skeletal muscle characteristics, transcriptomic profiles, and metabolic rates at rest and during exercise.
  • Evaluated cardiac regeneration capacity after ventricle amputation.

Main Results:

  • Mb deficiency caused tissue-specific transcriptomic changes, including altered respiration and differentiation pathways in the heart and skeletal muscle.
  • Mb knockout zebrafish showed reduced resting metabolic rate, enhanced hypoxia tolerance, and increased maximal swimming speed.
  • Mb expression was transiently suppressed during cardiac regeneration, suggesting a role in maintaining cardiomyocyte differentiation.

Conclusions:

  • Myoglobin loss in zebrafish induces coordinated transcriptomic shifts and impacts physiological functions.
  • Myoglobin plays a role in regulating metabolic rate, enhancing hypoxia tolerance, and potentially facilitating cardiac regeneration.