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Updated: Jan 13, 2026

A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion
Published on: February 2, 2021
FGA Serves as a Potential Diagnostic Marker and Therapeutic Target for Elderly Acute Kidney Injury
Hong Yu1, Jiacen Dai2, Shuping Deng3
1Department of Urology, Wuhan Third Hospital and Tongren Hospital of Wuhan University, Wuhan 430060, China.
Abstract:
To identify potential biomarkers and explore the underlying mechanisms of elderly acute kidney injury (e-AKI), we performed integrative plasma proteomics analysis on samples from 20 e-AKI patients and 20 age-matched non-AKI controls. Differential expression gene analysis, GSEA, WGCNA, random forest, and LASSO models were employed to identify hub genes, coupled with immune cell infiltration and clinicopathological correlation analyses. A renal ischemia-reperfusion injury mouse model validated key genes at protein and mRNA levels, while in vitro experiments explored the pathway involvement. We identified 229 e-AKI-associated genes enriched in immune, inflammatory, and coagulation pathways. Machine learning combined with the Nephroseq database yielded three hub genes; in vivo and in vitro experiments confirmed fibrinogen alpha chain (FGA) as the most relevant gene, which may regulate e-AKI progression via the cAMP/PKA/CREB pathway. Collectively, FGA holds promise as a diagnostic biomarker and therapeutic target for e-AKI, laying the theoretical foundation for its mechanistic research.
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