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Core-shell Cu@Pt nanoparticles and L-cysteine synergistically boosted electrochemical detection for wide-range
Jia-Qi Lyu1, Jiang-Tao Xu1, Hui-Ting Hu1
1School of Life and Environmental Sciences, Guangxi Human Physiological Information Non Invasive Detection Engineering Technology Research Center, Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent Medical Engineering for Chronic Diseases, Guangxi Colleges and Universities Key Laboratory of Biomedical Sensors and Intelligent Instruments, Guilin University of Electronic Technology, Guilin 541004, PR China.
Abstract:
An electrochemical detection method was proposed for the detection of Melatonin (MT) based on the synergistic signal amplification of L-Cys and core-shell Cu@Pt bimetallic nanoparticles (Cu@PtNPs). The successful fabrication of the PL-Cys/Cu@PtNPs/GCE was confirmed by characterization techniques including SEM, XRD, and TEM. The method exhibited excellent analytical performance, with a low detection limit of 0.03 μmol/L and a wide linear range across two concentration intervals: 0.1-100.0 μmol/L and 100.0-900.0 μmol/L. The method's practical applicability was successfully validated in human serum and various food samples, achieving satisfactory recovery rates of 98.73 %-99.67 % and 99.49 %-108.57 %, respectively, with relative standard deviations below 6.85 %. The novelty of this work lies in the synergistic amplification effect of the Cu@PtNPs and L-Cys and the application of density functional theory (DFT) calculations to elucidate the MT electrooxidation mechanism, confirming a 2-electron, 1-proton transfer process.
