Related Experiment Video
Updated: Jul 22, 2026

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
Published on: June 2, 2023
Different modified polysaccharides from Tremella fuciformis and its protective effects on oxidative damage
Chenglong Deng1, Jiaxin Duan1, Caihong Cheng1
1Hebei Key Laboratory of Natural Products Activity Components and Function, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004, China; Hebei Collaborative Innovation Center of Chestnut Industry, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004, China; Ministry of Education Engineering Research Center of Chestnut Industry Technology, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066004, China.
None:
Investigating the structure of polysaccharides enables a better understanding of the structure-activity relationship. Different modification methods help to improve the biological activities of polysaccharides. When different functional groups were introduced into polysaccharides, the activity changes of the derivatives obtained will be different. The ultrasonic assisted-H₂O₂/VC-three-phase partitioning (TPP) extraction coupled with high-pressure membrane filtration system was utilized to prepare low molecular weight (Mw) polysaccharides from Tremella fuciformis. These were then subjected to various modifications, resulting in sulfated T. fuciformis polysaccharides (SLTP), carboxymethylated (CLTP), phosphorylated (PLTP), and acetylated (ALTP). FT-IR spectroscopy verified the successful incorporation of desired functional groups. The measured degrees of substitution (DS) for SLTP, PLTP, CLTP, and ALTP were found to be 0.971, 0.803, 0.475, and 0.313, respectively. SEM imaging revealed that ALTP possessed a unique porous morphology. The modified polysaccharides demonstrated the ability to activate immune responses in RAW264.7 cells, with PLTP showing the strongest enhancement of nitric oxide (NO) production. Moreover, the modified polysaccharides improved cellular antioxidant defenses and reduced oxidative injury by an oxidative stress model. These results offer valuable guidance for the advanced utilization of T. fuciformis polysaccharides in food applications.
More Related Videos
07:40Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Published on: May 26, 2023
10:03Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
Published on: August 15, 2025
Related Concept Videos
Frequency-dependent Selection
Predator-Prey Interactions
Hybrid Zones
Mixtures of Acids
A Mixture of a Strong Acid and a Weak Acid
In a mixture of a strong acid and a weak acid, the strong acid dissociates completely and becomes a source of almost all the hydronium ions...
Acid Attack on Concrete
The rate at which hydrogen...