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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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Related Experiment Video

Updated: Jan 13, 2026

Assays for Validating Histone Acetyltransferase Inhibitors

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

Complex-specific inhibitors for interrogating ATAC histone acetyltransferase complex.

Sha Liu¹, Jinzhao Liu², Yinqiao Wu¹

¹Department of Chemistry, The University of Hong Kong, Hong Kong, China.

Nature Chemical Biology

|January 9, 2026

Summary

This summary is machine-generated.

Researchers developed a chemical inhibitor targeting the YEATS2 subunit to specifically disrupt the ATAC (Ada-two-A-containing) histone acetyltransferase complex. This approach suppressed tumor growth by reducing ATAC complex activity and gene expression.

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Last Updated: Jan 13, 2026

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Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Published on: August 10, 2017

Area of Science:

Biochemistry
Molecular Biology
Cancer Research

Background:

Histone acetyltransferases (HATs) regulate gene expression through chromatin modification.
HATs function in diverse protein complexes, influencing their specificity and function.
Understanding complex-specific roles of HATs is crucial for targeted therapies.

Purpose of the Study:

To develop a chemical method for specifically dissociating the ATAC HAT complex from chromatin.
To investigate the therapeutic potential of inhibiting the ATAC complex in cancer.

Main Methods:

Developed chemical inhibitors targeting YEATS2, a subunit specific to the ATAC complex.
Utilized LS-170, a potent inhibitor, to reduce ATAC complex chromatin occupancy.
Assessed the impact of ATAC complex inhibition on histone acetylation and gene expression.

Main Results:

LS-170 specifically decreased ATAC complex binding to chromatin.
Inhibition of the ATAC complex reduced histone acetylation levels.
Downregulation of ATAC-governed genes led to suppressed tumor growth in a lung cancer model.

Conclusions:

The ATAC HAT complex plays a significant role in gene transcription.
Chemical inhibition of the ATAC complex is a viable therapeutic strategy for cancer treatment.