Related Experiment Video

Updated: Jan 13, 2026

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

39.4K

PTG-Dependent Glycogen Metabolic Dysfunction Drives Impaired Adipose Browning: A Novel Mechanism Linking PM2.5 to

Limin Wang1,2, Renjie Hu1,2, Yanxi Chai1,2

  • 1School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|January 10, 2026
PubMed

Insights

Fine particulate matter (PM2.5) impairs adipose tissue browning by disrupting glycogen metabolism via protein targeting to glycogen (PTG). Restoring PTG or activating the ADRB3 receptor counteracts these effects, offering metabolic health targets.

Area of Science:

  • Environmental Health
  • Metabolic Science
  • Adipose Tissue Biology

Background:

  • Fine particulate matter (PM2.5) is linked to metabolic dysfunction.
  • The specific impact of PM2.5 on adipose tissue browning and the underlying mechanisms are not fully understood.

Purpose of the Study:

  • To investigate how PM2.5 affects adipose tissue browning.
  • To elucidate the molecular mechanisms, including the role of protein targeting to glycogen (PTG), involved in PM2.5-induced metabolic dysfunction.

Main Methods:

  • Exposure of mice to PM2.5.
  • Analysis of inguinal white adipose tissue (iWAT) browning, glycogen metabolism, and mitochondrial function.
  • Genetic manipulation of PTG and vascular endothelial growth factor B (VEGFB) levels.
  • Pharmacological activation of the β3-adrenergic receptor (ADRB3).

Main Results:

  • PM2.5 exposure inhibited iWAT browning by downregulating PTG and disrupting glycogen homeostasis.
  • Overexpression of PTG reversed PM2.5-induced impairments in browning, thermogenesis, and metabolic disorders.
  • PTG negatively regulated VEGFB; VEGFB knockdown rescued browning.
  • ADRB3 activation mitigated PM2.5 effects by restoring PTG and normalizing VEGFB.

Conclusions:

  • The ADRB3-PTG-VEGFB axis is central to PM2.5-induced metabolic dysfunction.
  • Adipose tissue glycogen metabolism is a key target for combating metabolic disruption caused by environmental factors like PM2.5.
Keywords:
fine particulate matterglycogen metabolismiWAT browningprotein targeting to glycogenvascular endothelial growth factor Bβ3‐adrenergic receptor

More Related Videos

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

2.2K
Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging08:31

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

Published on: July 1, 2021

3.5K

Related Concept Videos

Inborn Errors of Metabolism01:20

Inborn Errors of Metabolism

Phenylketonuria (PKU) is a protein metabolism disorder characterized by high blood levels of the amino acid phenylalanine. This results from a mutation in the gene responsible for phenylalanine hydroxylase, an enzyme that converts phenylalanine into tyrosine. When this enzyme is deficient, phenylalanine builds up in the blood, leading to symptoms such as vomiting, rashes, seizures, growth deficiency, and severe mental retardation. An early diagnosis and a diet restricting phenylalanine intake...
691
Overview of Carbohydrate Metabolism01:19

Overview of Carbohydrate Metabolism

Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
3.2K
Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
4.8K