Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Product Performance: In Vitro–In Vivo Correlation01:20

Drug Product Performance: In Vitro–In Vivo Correlation

226
In pharmaceutical development, it's crucial to establish a predictive in vitro–in vivo correlation (IVIVC) for two or more formulations to gain a comprehensive understanding of release properties. IVIVC reduces the need for costly in vivo studies and facilitates the establishment of meaningful dissolution specifications with significant cost savings and decreased regulatory burden. Furthermore, a meaningful IVIVC should predict Cmax and AUC within 20%, aligning with FDA guidance while...
226

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enabling the synthesis of multi-payload thio-antibody conjugates through the use of pyridazinediones, <i>p</i>-anisidine derivatives and various click chemistries.

RSC chemical biology·2026
Same author

Derivation of AZD5335, a Novel FRα-Targeted TOP1i-Loaded ADC, for the Treatment of FRα-Expressing Cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025
Same author

Post-stroke butyrate treatment shows sex-dependent microglial responses but does not improve outcomes in a mouse model of endothelin-1 sensory motor stroke.

BMC neuroscience·2025
Same author

Structure-guided design and development of cyclic peptide allosteric activators of Polycomb Repressive Complex 2.

bioRxiv : the preprint server for biology·2025
Same author

Extensive Biotransformation Profiling of AZD8205, an Anti-B7-H4 Antibody-Drug Conjugate, Elucidates Pathways Underlying Its Stability <i>In Vivo</i>.

Analytical chemistry·2024
Same author

Mediation of antitumor activity by AZD4820 oncolytic vaccinia virus encoding IL-12.

Molecular therapy. Oncology·2024
Same journal

RETRACTED: Sabir et al. DNA Based and Stimuli-Responsive Smart Nanocarrier for Diagnosis and Treatment of Cancer: Applications and Challenges. <i>Cancers</i> 2021, <i>13</i>, 3396.

Cancers·2026
Same journal

Correction: Adeluola et al. Chemoprevention of 4-NQO-Induced Oral Cancer by the Combination of Resveratrol and EGCG: In Vivo, In Silico and In Vitro Studies. <i>Cancers</i> 2026, <i>18</i>, 1098.

Cancers·2026
Same journal

Correction: Peñalver et al. Guidelines for Diagnosis, Treatment, and Follow-Up of Patients with Follicular Lymphoma-Spanish Lymphoma Group (GELTAMO) 2026. <i>Cancers</i> 2026, <i>18</i>, 395.

Cancers·2026
Same journal

Correction: Accorsi Buttini et al. Development of a Simplified Geriatric Score-4 (SGS-4) to Predict Outcomes After Allogeneic Hematopoietic Stem Cell Transplantation in Patients Aged over 50. <i>Cancers</i> 2025, <i>17</i>, 3278.

Cancers·2026
Same journal

Age-Stratified Long-Term Outcomes of Immune Checkpoint Inhibitors for Stage IV Melanoma and NSCLC in The Netherlands: A Population-Based Study.

Cancers·2026
Same journal

Targeting Ferroptosis in Glioblastoma: Molecular Mechanisms, Tumor Microenvironment, and Therapeutic Opportunities.

Cancers·2026
See all related articles

Related Experiment Video

Updated: Jan 13, 2026

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
07:25

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

Published on: May 4, 2017

18.2K

Integrating In Vitro Analytics for Improved Antibody-Drug Conjugate Candidate Selection.

Virginia Del Solar1, Ali Saleh1, Annarita Di Tacchio1

  • 1Oncology Targeted Discovery-Drug CandidateDC, Chemistry, AstraZeneca, London E1 2AX, UK.

Cancers
|January 10, 2026
PubMed
Summary
This summary is machine-generated.

Advanced analytical methods and computational tools accelerate antibody-drug conjugate (ADC) development. Integrating in vitro stability assays with data analysis improves candidate selection for clinical success.

Keywords:
ADC serum stabilityAntibody–drug conjugatescomputational integrationenzymatic assay

More Related Videos

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

15.2K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.1K

Related Experiment Videos

Last Updated: Jan 13, 2026

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
07:25

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

Published on: May 4, 2017

18.2K
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

15.2K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.1K

Area of Science:

  • Biopharmaceutical Development
  • Drug Discovery and Design
  • Analytical Chemistry

Background:

  • Antibody-drug conjugate (ADC) development faces challenges in conjugation chemistry, linker stability, and analytical controls.
  • Early screening and characterization of ADC candidates require advanced analytical methods, including plasma stability assays and enzymatic studies.
  • Integrating in vitro assays with data analysis software is crucial for assessing structure-activity relationships and identifying stable compounds.

Purpose of the Study:

  • To examine how combined analytical and computational approaches enhance ADC candidate selection.
  • To provide insights into the metabolic fate and stability risks of ADCs.
  • To demonstrate the predictive power of early-stage analytical studies for in vivo performance.

Main Methods:

  • Utilizing combined analytical and computational approaches for ADC candidate assessment.
  • Performing in vitro plasma stability assays and enzymatic studies.
  • Employing data analysis software for visualization and assessment of structure-activity relationships.

Main Results:

  • Demonstrated correlation between in vitro stability profiles and in vivo pharmacokinetic (PK) data.
  • Showcased the predictive power of early-stage analytical studies in ADC development.
  • Enabled faster, data-informed decision-making through software-driven analysis, streamlining candidate prioritization.

Conclusions:

  • Integrated in vitro analytics and computational tools are critical for efficient ADC development.
  • These integrated approaches support the selection of candidates with high clinical success potential.
  • Facilitates an accelerated and more effective path from discovery to clinical application for ADCs.