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CRISPR-Cas9 Gene Editing in Aspergillus: From Pathogenesis to Metabolic Engineering
Danni Hu1, Ruoyu Zhao1, Yingxu Lin1
1Jiangxi Key Laboratory of Natural Microbial Drug Research, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China.
Biology
|January 10, 2026
Summary
The CRISPR-Cas9 system offers precise gene editing in Aspergillus fungi, advancing research in pathogenicity and industrial biotechnology. This review details its mechanism and applications for Aspergillus functional genomics and cell factory development.
Area of Science:
- Microbiology
- Molecular Biology
- Biotechnology
Background:
- The genus Aspergillus includes over 600 filamentous fungi species with significant impacts on health, food, and industry.
- Efficient gene editing is vital for Aspergillus functional genomics and developing industrial cell factories.
Purpose of the Study:
- To provide an overview of the CRISPR-Cas9 system's composition and mechanism.
- To review the applications of CRISPR-Cas9 in Aspergillus species for research and biotechnology.
Main Methods:
- Detailed explanation of the CRISPR-Cas9 system, including Cas9 protein and PAM sequences.
- Review of CRISPR-Cas9 applications in pathogenic and industrial Aspergillus strains.
Main Results:
- CRISPR-Cas9 enables precise genetic modification in Aspergillus.
- Applications include pathogenicity attenuation, metabolic engineering, and functional genomics.
Conclusions:
- CRISPR-Cas9 technology is a powerful tool for Aspergillus research and industrial applications.
- Future opportunities lie in advancing Aspergillus industrial biotechnology through CRISPR-Cas9.
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