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Establishing Reagent Testing Platforms for Functional Analyses in Sunflower.

Ryan A Nasti1, Cathy S Kenderski2, Aryaa Chanchani2

  • 1Department of Plant & Microbial Biology, University of California, Berkeley, CA 94720, USA.

Plants (Basel, Switzerland)
|January 10, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed new methods for delivering genetic engineering tools in sunflowers. These platforms enable functional testing, advancing genetic modification for this important crop.

Keywords:
Helianthus annuusleaf infiltrationprotoplast transfectionsunflowertissue co-culturetransformation

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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Crop Science

Background:

  • CRISPR-based technologies offer advanced plant genome editing.
  • Sunflower (Helianthus annuus) lacks efficient transformation and transient reagent delivery systems.
  • Functional screening and validation of genetic engineering tools are hindered in sunflowers.

Purpose of the Study:

  • To establish and assess reagent delivery platforms for sunflower seedlings.
  • To compare the efficiency and scalability of different delivery methods.
  • To identify optimal components for transient functional testing in sunflowers.

Main Methods:

  • Systematic adaptation and assessment of protoplast transfection, leaf infiltration, and Agrobacterium-mediated tissue co-culture.
  • Comparison of different Agrobacterium strains and reporter gene expression cassettes.
  • Evaluation of reagent delivery efficiency and scalability in sunflower seedlings.

Main Results:

  • All three platforms (protoplast transfection, leaf infiltration, Agrobacterium-mediated co-culture) enabled successful reagent delivery in sunflowers.
  • Methods varied in scalability and efficiency.
  • Optimal Agrobacterium strains and expression cassettes were identified for specific applications.

Conclusions:

  • A foundational toolkit for transient functional testing in sunflower has been established.
  • These methods pave the way for advanced genetic engineering in Helianthus annuus.
  • This research supports the development of improved sunflower varieties for agricultural and horticultural purposes.