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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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Published on: June 20, 2019

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CRISPR/CAS9-based gene editing in cancer therapy: A systematic review and meta-analysis on current status and future

Shafee Ur Rehman1, Ghulam Husain Abbas1,2

  • 1Faculty of Medicine, Ala-Too International University, Bishkek, Kyrgyzstan.

Medicine
|January 10, 2026
PubMed
Abstract

Insights

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing shows promise for cancer therapy by targeting oncogenes and tumor suppressor genes. Further research is needed to address challenges like off-target effects for clinical translation.

Area of Science:

  • Biotechnology
  • Genetics
  • Oncology

Background:

  • CRISPR/Cas9 technology is a novel gene-editing tool with expanding applications in cancer therapy.
  • This review assesses the current and future potential of CRISPR/Cas9 in cancer treatment.

Purpose of the Study:

  • To review and meta-analyze the efficacy and challenges of CRISPR/Cas9 based gene editing in cancer treatment.
  • To explore the future directions and clinical translation of CRISPR/Cas9 technology in oncology.

Main Methods:

  • A comprehensive literature search was conducted in PubMed from 2015 to 2025.
  • 89 relevant studies were identified and analyzed for study design, CRISPR/Cas9 targets, delivery methods, therapeutic efficacy, and limitations.

Main Results:

  • CRISPR/Cas9 mediated disruption of oncogenes or restoration of tumor suppressor genes demonstrated significant tumor regression in preclinical studies.
  • The review evaluated the efficacy of CRISPR/Cas9 in targeting oncogenes, tumor suppressor genes, and immune modulation.
  • Analysis included off-target effects and integration with immunotherapy strategies.

Conclusions:

  • CRISPR/Cas9 is a highly promising tool for cancer gene editing and therapy.
  • Limitations such as off-target effects, delivery systems, and ethical considerations require resolution for improved clinical significance.
  • Further advancements are necessary to fully realize the clinical potential of CRISPR/Cas9 in cancer treatment.
Keywords:
CRISPR/Cas9cancer therapyclinical trialsimmunotherapytumor suppressor

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