Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coronavirus01:29

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimising DNA origami assembly by reducing off-target interactions.

Nature communications·2026
Same author

Artificial Biology - Assemble, Imitate, Adapt.

Advanced biology·2025
Same author

DNA Meets Protein─Development, Characterization, and Application of Aptamers against Peanut Allergen.

Journal of agricultural and food chemistry·2024
Same author

A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein.

International journal of molecular sciences·2024
Same author

Modular Approach for Rapid Identification of RNA-Based Sensors.

ACS sensors·2024
Same author

An RNA Motif That Enables Optozyme Control and Light-Dependent Gene Expression in Bacteria and Mammalian Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024

Related Experiment Video

Updated: Jun 16, 2026

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
07:44

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Published on: August 12, 2021

3.6K

Selection and Characterization of SARS-CoV-2 Spike Binding Clickmers.

Nima Moradzadeh1, Anna Jonczyk1, Anton Schmitz1

  • 1Life & Medical Sciences (LIMES) Institut, Chemical Biology & Medicinal Chemistry Unit, Universität Bonn, Gerhard-Domagk-Str. 1, 53121, Bonn, Germany.

Chembiochem : a European Journal of Chemical Biology
|January 10, 2026
PubMed
Summary

Chemically modified DNA aptamers called clickmers were developed to target the SARS-CoV-2 spike protein. These clickmers show high affinity for multiple variants, including Omicron, advancing diagnostic potential.

Keywords:
Covid‐19SARS‐CoV‐2 spike proteinaptamerschemically modified aptamersclickmers

More Related Videos

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
06:39

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Published on: April 21, 2022

3.5K
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

1.2K

Related Experiment Videos

Last Updated: Jun 16, 2026

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
07:44

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Published on: August 12, 2021

3.6K
High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
06:39

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Published on: April 21, 2022

3.5K
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

1.2K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Expanding the chemical diversity of aptamers is crucial for enhancing their diagnostic capabilities.
  • Aptamers offer a promising alternative to antibodies for molecular recognition applications.

Purpose of the Study:

  • To develop chemically modified DNA aptamers (clickmers) targeting the SARS-CoV-2 spike glycoprotein using click-SELEX.
  • To evaluate the binding affinity and specificity of generated clickmers against various SARS-CoV-2 variants.

Main Methods:

  • Click-systematic evolution of ligands by exponential enrichment (SELEX) was employed to generate clickmers.
  • Binding affinities were assessed using flow cytometry, surface plasmon resonance (SPR), and microscale thermophoresis (MST).
  • Structure-function analysis identified key modification sites and evaluated truncated variants.

Main Results:

  • Two distinct clickmer families targeting SARS-CoV-2 spike protein were generated, functionalized with benzofuran or indole moieties.
  • Lead candidates (BF1 and N2) exhibited nanomolar affinity for wild-type SARS-CoV-2 spike protein.
  • Clickmers demonstrated maintained binding to Alpha, Delta, and Mu variants, with a truncated N2 variant showing enhanced binding to Omicron.

Conclusions:

  • The click-SELEX platform is versatile for generating chemically modified aptamers against clinically relevant targets.
  • Developed clickmers show potential for SARS-CoV-2 diagnostics, with promising affinity and variant coverage.
  • Truncated clickmer variants can retain or enhance binding, offering opportunities for optimized aptamer design.