Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Flora of the Large Intestine01:29

Bacterial Flora of the Large Intestine

1.5K
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trade-offs across life history stages and social association types shape winter communal roosting in a long-lived raptor.

The Journal of animal ecology·2025
Same author

How carton-nest fungi of the ant <i>Lasius fuliginosus</i> interact with each other and with the root-rot fungus <i>Armillaria mellea</i>.

Mycologia·2025
Same author

Bacterial communities of wild bee species and the western honey bee (Apis mellifera) (Hymenoptera: Apoidea): Alpine insights.

Journal of insect science (Online)·2025
Same author

A Comparative Study of Effects of Biodegradable and Non-biodegradable Microplastics on the Growth and Development of Black Soldier Fly Larvae (<i>Hermetia illucens</i>).

Waste and biomass valorization·2024
Same author

Evolutionary history of an Alpine Archaeognath (<i>Machilis pallida</i>): Insights from different variant.

Ecology and evolution·2023
Same author

Expanding the Pathogen Panel in Wastewater Epidemiology to Influenza and Norovirus.

Viruses·2023

Related Experiment Video

Updated: Jan 13, 2026

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
09:34

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Published on: July 30, 2016

22.1K

Stage-Specific Microbiota Transitions Throughout Black Soldier Fly Ontogeny.

Thomas Klammsteiner1, Carina D Heussler2, Katharina T Stonig2

  • 1Department of Ecology, Universität Innsbruck, Technikerstr. 25, 6020, Innsbruck, Austria. thomas.klammsteiner@uibk.ac.at.

Microbial Ecology
|January 10, 2026
PubMed
Summary

The black soldier fly (BSF) converts waste into protein. Its development is influenced by bacteria, with distinct microbial shifts occurring before egg-laying, impacting future insect generations.

Keywords:
AttractantsCircular economyInsect farmingInsect immunityInterkingdom communicationMicrobial colonization

More Related Videos

Author Spotlight: Axenic Rearing of Delia antiqua to Detect the Impact of Intestinal Microbes on Its Growth and Development
05:58

Author Spotlight: Axenic Rearing of Delia antiqua to Detect the Impact of Intestinal Microbes on Its Growth and Development

Published on: December 22, 2023

1.0K
Author Spotlight: Exploring Bradysia coprophila's Unique Biology &#8211; A Guide to Laboratory Maintenance
04:26

Author Spotlight: Exploring Bradysia coprophila's Unique Biology – A Guide to Laboratory Maintenance

Published on: April 19, 2024

1.7K

Related Experiment Videos

Last Updated: Jan 13, 2026

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
09:34

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Published on: July 30, 2016

22.1K
Author Spotlight: Axenic Rearing of Delia antiqua to Detect the Impact of Intestinal Microbes on Its Growth and Development
05:58

Author Spotlight: Axenic Rearing of Delia antiqua to Detect the Impact of Intestinal Microbes on Its Growth and Development

Published on: December 22, 2023

1.0K
Author Spotlight: Exploring Bradysia coprophila's Unique Biology &#8211; A Guide to Laboratory Maintenance
04:26

Author Spotlight: Exploring Bradysia coprophila's Unique Biology – A Guide to Laboratory Maintenance

Published on: April 19, 2024

1.7K

Area of Science:

  • Insect microbiology
  • Sustainable protein production
  • Waste management

Background:

  • Growing global protein demand and waste management challenges necessitate alternative solutions.
  • The black soldier fly (BSF; Hermetia illucens) offers a dual solution by converting organic waste into valuable protein.
  • Microbial communities significantly influence BSF development, behavior, and reproductive success, particularly oviposition.

Purpose of the Study:

  • To investigate the microbial communities throughout the BSF life cycle.
  • To specifically characterize the egg surface microbiota and trace its origin.
  • To understand how microbial composition changes during BSF maturation and before oviposition.

Main Methods:

  • Analysis of bacterial communities in various BSF life stages: larval haemolymph and gut, pupal pulp, ovipositor wash, eggs (ovarian, post-oviposition, surface-sterilized), and adult abdomen.
  • Comparison of microbiota in larvae raised on sterilized versus non-sterilized feed.
  • 16S rRNA gene sequencing to profile bacterial communities.

Main Results:

  • Distinct bacterial profiles were observed across different BSF life stages.
  • Larval stages showed dominance of Enterobacteriaceae (e.g., Morganella, Escherichia, Proteus).
  • Egg surfaces were predominantly colonized by Burkholderiaceae (Burkholderia-Caballeronia-Paraburkholderia), indicating a microbial transition before oviposition.

Conclusions:

  • Microbial communities undergo significant transformation during BSF maturation, especially preceding oviposition.
  • While some dominant bacteria persist, the overall community composition shifts, influencing egg development.
  • Understanding these microbial dynamics, particularly egg surface microbiota, can optimize BSF rearing for enhanced protein production and waste management.