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Related Concept Videos

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Related Experiment Video

Updated: Jan 13, 2026

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
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Cleavage Under Targets and Tagmentation (CUT&Tag) Protocol for Plants.

Xiao-Yuan Tao1, Jia-Wei Wang2,3,4,5

  • 1Xianghu Laboratory, Hangzhou, China. taoxiaoyuan@xhlab.ac.cn.

Methods in Molecular Biology (Clifton, N.J.)
|January 10, 2026
PubMed
Summary

Cleavage Under Targets and Tagmentation (CUT&Tag) offers efficient chromatin profiling with less input material and higher signal than ChIP. This study details a CUT&Tag protocol for plant cells and reviews related methods.

Keywords:
B-CUT&TagCUT&TagMultimodalNano-CUT&TagPlantSingle-cell

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Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Chromatin profiling is crucial for understanding gene regulation.
  • Traditional methods like ChIP have limitations in input requirements and signal-to-noise ratio.
  • Enzyme-tethering strategies offer improved chromatin analysis.

Purpose of the Study:

  • To provide a detailed protocol for bulk-cell CUT&Tag in plants.
  • To introduce derivative CUT&Tag methods for broader applications.
  • To optimize chromatin profiling in plant systems.

Main Methods:

  • Developed a detailed protocol for Cleavage Under Targets and Tagmentation (CUT&Tag) in plant cells.
  • Utilized an enzyme-tethering strategy for targeted DNA fragmentation.
  • Adapted and validated the method for bulk-cell analysis.

Main Results:

  • The CUT&Tag method requires a smaller amount of cell/nuclei input compared to ChIP.
  • Generated data exhibits a higher signal-to-noise ratio.
  • Successfully applied CUT&Tag for profiling histone modifications and protein-DNA interactions in plants.

Conclusions:

  • CUT&Tag is a powerful and efficient technique for plant chromatin profiling.
  • The described protocol facilitates wider adoption of CUT&Tag in plant epigenetics research.
  • Derivative CUT&Tag methods expand the utility of this approach.