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JULGI coordinates vascular development and leaf patterning through non-cell-autonomous regulation of miR165/166
Soyoung Park1, Hyun Seob Cho1, Seungchul Lee1
1Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea.
None:
The interplay between vascular development and leaf development is central to the evolutionary success of vascular plants, yet the underlying mechanisms remain poorly understood. Here, we identify the RNA-binding protein JULGI1 (JUL1) as a key integrator of vascular and leaf development in Arabidopsis. JUL1 is transcribed in leaf veins, while the protein distributes throughout the leaf, where it binds G-rich regions of pri-MIR165a transcripts on the abaxial side. This interaction disrupts hairpin formation and inhibits DICER-LIKE1 (DCL1)-mediated processing, reducing miR165/166 levels. Consequently, transcript levels of their targets, the Class III homeodomain leucine zipper (HD-ZIP III), increase, reinforcing adaxial-abaxial polarity. Our findings reveal a non-cell-autonomous mechanism by which vascular-derived JUL1 modulates miRNA biogenesis to coordinate leaf patterning with vascular development.
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