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Updated: Jan 13, 2026
Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
Published on: March 21, 2019
Optimization of TX-100/SDS-based decellularized vascular material using ultrasound and chemical treatment: evaluation
Hongguang Chen1, Xiaomei Bie2, Lifang Hao3
1Department of Orthopedics, the Fourth Medical Center of PLA General Hospital, Beijing, People's Republic of China.
Abstract:
Decellularized blood vessels with low immunogenicity and excellent biocompatibility are promising for tissue engineering and clinical applications. However, current decellularization methods face limitations in cell removal efficiency, matrix preservation, and biosafety. This study optimized the Triton X-100/SDS (TX-100/SDS) decellularization method using ultrasound technology by systematically evaluating the effects of ultrasound power, temperature, and processing time on decellularization efficiency. The optimized method achieved a 72% reduction in nucleic acid residues at 100 W power while preserving matrix integrity and significantly reducing chemical reagent residues. Structural and biosafety evaluations confirmed that the optimized scaffolds met biological safety standards and demonstrated excellent stability, providing a strong foundation for developing high-performance decellularized vascular materials for clinical applications.
