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Updated: Jan 13, 2026

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
VvmiR408 positively modulates seedlessness formation by cleaving VvAGL61 in grape berries
Zimeng Yao1, Hui Yang1, Yumiao Wang1
1College of Horticulture, Nanjing Agricultural University, Nanjing, 211800, People's Republic of China.
None:
Grape seedlessness, a pivotal agronomic trait, is governed by intricate molecular mechanisms that remain partially elucidated. In this study, we identified VvmiR408 as a key regulator targeting VvAGL61 (a class D MADS-box gene) through RLM-RACE and PPM-RACE analysis, with cleavage validation at the 10th nucleotide of the miR408 complementary site, and uncover VvmiR408-VvAGL61 is a pivotal regulatory module during grape seed development, with a striking antagonism of spatial-temporal expression profiles between VvmiR408 and VvAGL61 in this process: VvmiR408 peaked during seed abortion in the seedless cultivar 'Jingkejing' (40 DAF, 3.8-fold increase), while VvAGL61 dominated during seed development in the seeded 'Wink' (40 DAF, 11-fold elevation), indicating VvmiR408 promoting seed abortion in seedless berries while VvAGL61 enhances seed development. This hypothesis was further validated by transgenic tobacco overexpressing VvMIR408, which exhibited a significant reduction in both seed number and silique size, through the down-regulation of NtAGL61, the tobacco ortholog of VvAGL61. Collectively, we propose an interaction model of the miR408-AGL61 module mediating seed development and abortion in grapes and tobaccos. This model highlights key target sites for molecular breeding aimed at producing high-quality seedless grapes.
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