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Epidemiological characterization and clinical utility of the multiplex PCR FilmArray™ Gastrointestinal Panel at a
Yoshitaka Asagai1, Hiroshi Yamaguchi1, Tomohiko Yamamura1
1Department of Pediatrics, Kobe University Graduate School of Medicine, Hyogo, Japan.
Background:
Rapid and reliable diagnostic methods are essential to ensure appropriate management of acute gastroenteritis. The BioFire FilmArray® Gastrointestinal Panel (FGP) rapidly and accurately identifies various pathogens. However, previous studies on FGP lacked detailed descriptions of its utility in clinical practice. Additionally, pediatric data from Japan are limited. Therefore, we aimed to evaluate the clinical utility of the FGP and identify the characteristics of patients with positive FGP results.
Methods:
We retrospectively reviewed the records of pediatric patients (aged ≤18 years) who underwent FGP testing at Kobe University Hospital in 2024. The pathogens detected and clinical characteristics of FGP-positive and FGP-negative patients were compared. Subgroup analyses were conducted by bacterial- and viral-positive status.
Results:
The median time from FGP testing to the confirmation of results was 82 min. Of the 67 patients who underwent FGP testing, 28 (42 %) were FGP-positive. The most frequently detected pathogen was norovirus (18 %), followed by Clostridioides difficile toxin A/B (12 %), Campylobacter spp. (4.5 %), Yersinia enterocolitica (4.5 %), Enteroaggregative Escherichia coli (4.5 %), Enteropathogenic Escherichia coli (3 %), astrovirus (3 %), sapovirus (1.5 %), Shiga -like toxin producing Escherichia coli (1.5 %), and Salmonella spp. (1.5 %). No significant differences in clinical findings were observed between FGP-positive and FGP-negative cases. Subgroup analysis revealed frequent vomiting, and significantly shorter fever in patients with viral-positive FGP results than in those with bacterial-positive FGP results.
Conclusion:
FGP aids clinical decision-making by enabling the early detection of pathogens, such as Campylobacter spp. and Yersinia enterocolitica, which typically require several days for stool culture results.
Insights
The BioFire FilmArray® Gastrointestinal Panel (FGP) offers rapid pathogen detection in pediatric gastroenteritis, aiding clinical decisions. Viral infections showed shorter fever durations compared to bacterial ones.
Area of Science:
- Clinical microbiology
- Pediatric infectious diseases
- Diagnostic technology evaluation
Background:
- Accurate diagnosis of acute gastroenteritis is crucial for effective patient management.
- The BioFire FilmArray® Gastrointestinal Panel (FGP) provides rapid pathogen identification.
- Limited pediatric data from Japan necessitates further evaluation of FGP utility.
Purpose of the Study:
- To assess the clinical utility of the FGP in a Japanese pediatric cohort.
- To characterize patients with positive FGP results.
- To compare clinical features between bacterial and viral gastroenteritis identified by FGP.
Main Methods:
- Retrospective review of pediatric patients (≤18 years) undergoing FGP testing.
- Comparison of clinical characteristics between FGP-positive and FGP-negative groups.
- Subgroup analysis based on bacterial vs. viral pathogen detection.
Main Results:
- FGP testing yielded results within a median of 82 minutes.
- 28 out of 67 (42%) pediatric patients tested positive for gastrointestinal pathogens.
- Norovirus was the most common pathogen; viral infections were associated with shorter fever duration than bacterial infections.
Conclusions:
- The FGP facilitates timely clinical decision-making by rapidly identifying key pathogens.
- Early detection of pathogens like Campylobacter spp. and Yersinia enterocolitica improves upon traditional stool culture methods.
- FGP analysis provides valuable insights into pediatric gastroenteritis epidemiology and clinical presentation.
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