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Related Experiment Video

Updated: Jan 13, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Rapid in-plant directed evolution with GRAPE.

Xiangtan Chen1, Rui Deng2, Youjun Zhang3

  • 1State Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China; School of Advanced Interdisciplinary Sciences, University of Chinese Academy of Sciences, Beijing, China.

Trends in Plant Science
|January 11, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed GRAPE (geminivirus replicon-assisted in planta directed evolution), a new platform for plant evolution. This method overcomes bottlenecks by linking replication to protein function, enabling high-throughput screening in plants.

Keywords:
GRAPE (geminivirus replicon-assisted in planta directed evolution)directed evolution in plantageminivirus replicon

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Plant directed evolution faces bottlenecks limiting throughput.
  • Existing methods struggle to maintain native plant signaling and defense pathways during selection.

Purpose of the Study:

  • Introduce geminivirus replicon-assisted in planta directed evolution (GRAPE) as a novel platform.
  • Overcome limitations in plant evolutionary engineering.
  • Enable high-throughput functional screening in plants.

Main Methods:

  • Utilized geminivirus replicons for in planta replication.
  • Linked rolling-circle replication (RCR) to protein function.
  • Employed replicon amplification for selection.

Main Results:

  • GRAPE successfully remediates plant-directed evolutionary bottlenecks.
  • Achieved microbe-like throughput for selection in planta.
  • Preserved native plant signaling and defense mechanisms during the process.

Conclusions:

  • GRAPE offers a powerful new tool for plant evolutionary biology and biotechnology.
  • The platform facilitates rapid functional screening and engineering of plant proteins.
  • This approach enhances the study and manipulation of plant systems.