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EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

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Related Experiment Video

Updated: Jan 13, 2026

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Published on: March 25, 2022

Unraveling the Morphological and Functional Maturation Mechanisms Underlying Human Neural Development Using

Yue Tian^1,2, Yi-Chun Ou¹, Zi-Xian Zhang¹

¹Department of Neurobiology, School of Basic Medical Sciences, Peking University Health Science Center and Neuroscience Research Institute, Peking University, Beijing, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|January 12, 2026

Summary

This study introduces a rapid human neuronal model from stem cells, revealing L-type calcium channels and ECEL1 are crucial for early brain development and neuronal maturation.

Keywords:

calmodulin 3 (CALM3)endothelin converting enzyme‐like 1 (ECEL1)hiPSCs‐derived neuron human induced pluripotent stem cells (hiPSCs)human neural development voltage‐gated calcium channels voltage‐gated sodium ion channels

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Published on: January 8, 2017

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Last Updated: Jan 13, 2026

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

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Published on: March 25, 2022

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Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Published on: January 8, 2017

Area of Science:

Neuroscience
Developmental Biology
Stem Cell Biology

Background:

Human induced pluripotent stem cells (hiPSCs) offer a model for neural development.
Current hiPSC-to-neuron differentiation protocols are time-consuming, hindering study of early neuronal morphogenesis and maturation.

Purpose of the Study:

To develop a rapid in vitro human neuronal model from hiPSCs.
To investigate the roles of L-type voltage-gated calcium channels (Cav1.2, Cav1.3) and ECEL1 in early human neuronal development.

Main Methods:

Utilized a combined small molecules and proteins (SMP) protocol for rapid hiPSC-derived neuron differentiation.
Analyzed the impact of SMP-induced neuronal models on studying early human neuronal development.

Main Results:

The SMP protocol yielded rapidly maturing hiPSC-derived neurons, recapitulating early human neuronal development.
Cav1.2 and Cav1.3 channels are essential for early human neuronal morphogenesis.
Endothelin converting enzyme-like 1 (ECEL1) regulates functional maturation via calmodulin 3 (CALM3), impacting ion channel expression.

Conclusions:

A novel, temporally compressed hiPSC-derived neuronal model accelerates the study of early human brain development.
Identified key molecular players, including Cav1.2, Cav1.3, ECEL1, and CALM3, in neuronal morphogenesis and functional maturation.