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The Calcium-Binding Protein S100A10 (p11) Is Required for Normal Motor Performance by Regulating Vesicle Dynamics at
Esther Vilches-Herrando1,2, Guillermo Rodríguez-Bey1,3, Rosendo G Hernández4
1GRUpo de NEuroDEgeneración y NeurorREparación (GRUNEDERE), Área de Fisiología, Facultad de Medicina, Universidad de Cádiz, Cádiz, Spain.
Acta Physiologica (Oxford, England)
|January 12, 2026
Summary
The protein p11 regulates neurotransmitter release by affecting vesicle dynamics at central excitatory synapses. This finding is crucial for understanding neural information processing and brain function.
Area of Science:
- Neuroscience
- Molecular Biology
- Synaptic Physiology
Background:
- Understanding neural information processing requires identifying key interactors in sensorimotor processing and neurotransmission.
- The precise role of p11 in these processes remains to be fully elucidated.
Purpose of the Study:
- To investigate the function of p11 in sensorimotor processing and excitatory synaptic neurotransmission.
- To identify interacting partners of p11 within the presynaptic active zone.
Main Methods:
- Utilized neuron-specific p11 silencing (lentivirus and siRNA) in rat hypoglossal motor neurons (HMNs).
- Employed techniques including western blot, co-immunoprecipitation, proximity ligation assays (PLA), electron microscopy, and whole-cell patch-clamp recordings.
- Analyzed AMPA receptor-mediated excitatory postsynaptic currents and vesicle dynamics.
Main Results:
- p11 knockdown reduced inspiratory activity in HMNs and depressed excitatory synaptic strength.
- p11 interacts with Munc13-1, a presynaptic active zone protein.
- p11 interference led to Munc13-1 downregulation, altered active zone morphology, and impaired vesicle release and replenishment.
Conclusions:
- p11 acts as a mediator of neurotransmitter release by regulating vesicle dynamics at central excitatory synapses.
- p11 is a multifaceted factor critical for neurotransmission, synaptic plasticity, and neural information processing.
Keywords:
S100A10motor neuronneurotransmitter releasereadily releasable pool (RRP)replenishment rate of RRPsynchronous and asynchronous releaseMore Related Videos
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