Activated Platelet and Platelet-Derived Microparticle Levels: A Comparative Study between Apheresis Platelet...

To prepare a therapeutic dose of platelets in adults, 4-6 platelet concentrate (PC) from whole blood or one unit of apheresis are required. This study aimed to evaluate the effect of the preparation process of apheresis-PCs and pooled-PCs from platelet-rich plasma (PRP) on the platelet microparticles (PMP) count and platelet activation during storage. This study used four apheresis-PCs from the Haemonetics MCS + and four pooled-PCs. Six PRP-PCs were pooled and filtered to prepare pooled PRP-PC. The quality of the PCs was measured by platelet count, pH, swirling, P-selectin expression (as a marker of platelet activation), and PMP count on days 1, 3, and 5. WBC and RBC count on the first day and bacterial contamination on day 5. The platelet count in the pooled PRP-PCs was significantly lower on the fifth day compared to the first day. The PMP count was significantly higher on day 5 compared to day 1 for the pooled PRP-PCs, for the apheresis-PCs, the PMP count was higher on day 3 and day 5 compared to day 1. The WBC and RBC count in the apheresis-PCs was significantly lower. The P-selectin expression on the first day in the apheresis-PCs was significantly higher. The PMP count in the apheresis-PCs was significantly higher than the pooled PRP-PCs on day 3. The pooled PRP-PCs met standard quality parameters like apheresis-PCs. Pooled PRP-PCs showed lower platelet activation and PMP compared to apheresis-PCs in the early storage, indicating potential for clinical use.