Hippocampal Beta-Amyloid Accumulation In Aluminium Chloride and D-Galactose-Induced Rats: Establishing a
Thirupathirao Vishnumukkala1,2, Samaila M Chiroma3, Saravanan Jagadeesan4
1Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
Journal of Pharmacy & Bioallied Sciences
|January 12, 2026
Summary
Researchers developed a new rat model for Alzheimer
Area of Science:
- Neuroscience
- Pathology
- Animal Models
Background:
- Alzheimer's disease (AD) involves tau protein and beta-amyloid (Aβ) plaques, causing cognitive decline.
- Existing nontransgenic models using aluminum chloride (AlCl3) and D-galactose (D-gal) partially mimic AD, but Aβ deposition in the hippocampus is not fully understood.
Purpose of the Study:
- To investigate beta-amyloid accumulation in the hippocampal CA2 region.
- To validate a nontransgenic rat model induced by combined AlCl3 and D-gal administration for Alzheimer's disease research.
Main Methods:
- Adult male Wistar rats were divided into control and model groups.
- The model group received daily oral AlCl3 and intraperitoneal D-gal for 10 weeks.
- Beta-amyloid expression in the hippocampal CA2 region was assessed using immunohistochemistry.
Main Results:
- Immunohistochemistry showed significant beta-amyloid immunoreactivity and deposits in the CA2 subfield of the model group.
- The control group exhibited minimal beta-amyloid staining, confirming the model's efficacy.
Conclusions:
- Combined AlCl3 and D-gal administration effectively induces beta-amyloid accumulation in the hippocampal CA2 region.
- This validated nontransgenic model is suitable for studying Alzheimer's disease pathogenesis and testing therapies.
