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Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
Published on: November 19, 2019
tRF-29-86J8WPMN1EJ3: a tRNA-derived small RNA promoting esophageal cancer progression
Xinnian Yu1, Zhipeng Li2, Yuqi Fu1
1Department of Medical Oncology, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing, China.
Background:
Transfer RNA-derived small RNAs (tsRNAs) have been shown to participate in tumorigenesis and progression of human cancers. We aimed to identify dysregulated tsRNAs in esophageal cancer (EC) and uncover the underlying mechanisms.
Methods:
tsRNA profiles for EC were downloaded from MINTbase. We also detected expression levels of tsRNAs in EC tissues using tsRNA sequencing and real-time quantitative polymerase chain reaction (RT-qPCR). The functional roles of tRF-29-86J8WPMN1EJ3 in EC were investigated by transfecting EC cell lines with mimics of tRF-29-86J8WPMN1EJ3 and assessing phenotypes through colony formation, wound healing, and Transwell assays.
Results:
Totally, 210 differentially expressed tsRNAs were identified from MINTbase. In addition, 40 EC patients were recruited, and four paired EC and adjacent normal tissues were randomly selected for tsRNA sequencing, identifying 159 dysregulated tsRNAs, which were intersected with the 210 tsRNAs from MINTbase, obtaining three tsRNAs: tRF-28-P4R8YP9LOND5, tRF-29-86J8WPMN1EJ3, and tRF-29-P4R8YP9LONHK. Their expression levels were validated in 40 paired tissues. All three tsRNAs were up-regulated in EC tissues, and their high expression levels were associated with shorter overall survival (OS) in EC patients. In vitro experiments indicated that overexpression of tRF-29-86J8WPMN1EJ3 could promote proliferation, migration, and invasion of EC cell lines.
Conclusions:
In conclusion, we presented the expression characteristics of tsRNAs in EC and identified three up-regulated tsRNAs in EC tissues. Lower expression levels of these tsRNAs were associated with better OS in EC patients. Additionally, tRF-29-86J8WPMN1EJ3 promotes the proliferation, migration, and invasion of EC cells.
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