Related Experiment Video
Updated: Jan 13, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
SarcGraph for High-Throughput Regional Analysis of Sarcomere Organization and Contractile Function in 2D Cardiac
Saeed Mohammadzadeh1, Yao-Chang Tsan2, Aaron Renberg2
1Department of Mechanical Engineering, Boston University, Boston, Massachusetts, United States.
None:
Timelapse images of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) provide rich information on cell structure and contractile function. The two-dimensional cardiac muscle bundle (2DMB) platform standardizes tissue geometry, enabling physiologic, uniaxial contractions on elastomeric substrates. However, larger sarcomere displacements in 2DMBs challenge existing tracking pipelines. We present adaptations to SarcGraph, an open-source Python package for sarcomere detection and tracking, enabling automated analysis of high-frame-rate 2DMB videos. Key modifications include frame-by-frame detection with automated segmentation, Gaussian Process Regression for denoising, and automatic contractile phase detection. We provide 130 example movies through Harvard Dataverse, enabling high-throughput analysis and advancing hiPSC-CM research.
More Related Videos
08:37Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
Published on: March 3, 2021
07:02Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
Published on: November 3, 2020
Related Concept Videos
The Sarcomere
Each...
Structure of Cardiac Muscles
Compared to skeletal muscles, cardiac muscle cells are small and mostly have a single nucleus. Additionally, they are usually...
Microscopic Anatomy of Skeletal Muscles
The muscle sarcolemma is a plasma membrane enclosing each muscle cell that conducts electrical signals called action potentials. The sarcolemma extends into the cell to form T-tubules, ensuring the neural impulses are uniformly distributed across the entire muscle...
Overview of Skeletal Muscle
Actin and Myosin in Muscle Contraction
Fascicle Arrangement in Skeletal Muscles
The four primary types of muscle based on fascicle arrangement are: