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Intronic Single Nucleotide Polymorphisms in FGFR2 Gene Association With Non-Syndromic Mandibular Retrognathism
Caio Luiz Bitencourt Reis1, Christian Kirschneck2, Daniel Hemming3
1School of Dentistry, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
Objective:
Mandibular retrognathism (MR) is a skeletal malocclusion in which patients have a deficient mandibular length, resulting in a more posterior position of the mandible. We aimed to investigate the association between Single nucleotide polymorphisms (SNPs) in Fibroblast Growth Factor Receptor 2 (FGFR2) gene and MR in germans.
Materials And Methods:
Genomic DNA and lateral cephalometric radiographs were obtained from orthodontic patients. Patients were allocated into the 'Retruded' group (SNB angle < 78°) and into the 'Well-positioned' group (SNB 78°-82°). The rs4752566, rs10736303, rs11200014, rs1078806, rs1219648, rs2981578 and rs2162540 SNPs were genotyped using real-time PCR. Allele, genotype and haplotype frequencies were compared (α = 5%).
Results:
A total of 142 patients were included, 93 (65.5%) allocated into the 'Retruded' group and 49 (34.5%) into the 'Well-positioned' group. The allele T in rs2981578 SNP was statistically more frequent in the 'Retruded' group in both univariate (PR = 1.22; 95% CI, = 1.02-1.47) and multivariate (PR = 1.55; 95% CI, = 1.07-2.25) analyses (p < 0.05). The CT + TT genotypes were statistically more frequent in the 'Retruded' group in univariate (PR = 1.58; 95% CI, = 1.03-2.41) and multivariate (PR = 1.59; 95% CI, = 1.11-2.26) analysis (p < 0.05). All studied SNPs were associated with MR establishment in haplotype analysis (p < 0.05).
Conclusion:
SNPs in the FGFR2 are associated with MR and have the potential to serve as genetic biomarkers to early diagnosis and prediction of mandible growth.
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