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Related Concept Videos

Long-patch Base Excision Repair01:02

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Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
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Base Excision Repair01:54

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One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
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Nucleotide Excision Repair01:38

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DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
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Related Experiment Video

Updated: Jan 13, 2026

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
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Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

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The PagDMG6341-PagWD40-PagPOLD4 Module Coordinates Base Excision Repair in '84K' Poplar (Populus alba × P.

Aoyu Ling1, Yijia Jin2, Yufei Xia1

  • 1State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.

Plant Biotechnology Journal
|January 12, 2026
PubMed
Summary

Scientists discovered a new scaffold protein, PagWD40, that links DNA repair proteins PagDMG6341 and PagPOLD4 in poplar plants. This finding advances our understanding of base excision repair (BER) mechanisms in plants.

Keywords:
DNA glycosylaseDNA polymeraseWD40‐like proteinbase excision repairscaffold protein

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Area of Science:

  • Plant Molecular Biology
  • DNA Repair Mechanisms
  • Biochemistry

Background:

  • Base excision repair (BER) is crucial for DNA base repair, but its protein interactions are poorly understood in plants.
  • Previous research has not fully elucidated the molecular players involved in plant BER pathway assembly.

Purpose of the Study:

  • To identify and characterize key proteins involved in the base excision repair pathway in poplar ('84K' poplar).
  • To investigate the interaction network and functional roles of identified BER-related genes in plants.

Main Methods:

  • Transcriptome analysis and weighted gene co-expression network analysis (WGCNA) were used to identify BER-responsive genes.
  • RNA interference (RNAi) was employed to assess gene function.
  • Yeast two-hybrid (Y2H), AlphaFold3 modeling, bimolecular fluorescence complementation (BiFC), and luciferase complementation imaging (LCI) assays were used to study protein interactions.

Main Results:

  • Two key BER-responsive genes, PagDMG6341 (DNA glycosylase) and PagPOLD4 (DNA polymerase δ subunit), were identified.
  • A scaffold protein, PagWD40, was found to interact with both PagDMG6341 and PagPOLD4, forming a functional complex.
  • PagWD40 acts as a molecular bridge, linking the DNA glycosylase and DNA polymerase in the plant BER pathway.

Conclusions:

  • A novel PagWD40-mediated scaffold mechanism is revealed for the plant base excision repair pathway.
  • This study provides new insights into the organization and regulation of DNA damage repair networks in plants.