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Mining a vibriophage depolymerase for enhanced pathogen control in aquaculture.

Yufei Yue1,2, Jiulong Zhao1,2,3,4,5, Zengmeng Wang1,2,4

  • 1Qingdao New Energy Shandong Laboratory, Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

Applied and Environmental Microbiology
|January 12, 2026
PubMed
Summary
This summary is machine-generated.

Phage depolymerases, like the novel Dep193, combat antibiotic-resistant Vibrio in aquaculture. Combining Dep193 with phage VnaP enhances bacterial clearance and delays resistance, offering a promising aquaculture therapy.

Keywords:
Dep193Vibrio controlantibiofilm activityphage synergyphage-derived depolymerasepolysaccharide degradation

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Area of Science:

  • Microbiology
  • Biotechnology
  • Aquaculture Science

Background:

  • Antibiotic resistance in *Vibrio* species poses a significant threat to global aquaculture.
  • Bacteriophages (phages) and their derived enzymes, particularly depolymerases, show promise as alternatives to antibiotics.
  • Phage depolymerases can degrade bacterial surface polysaccharides, potentially enhancing antibacterial efficacy and overcoming resistance.

Purpose of the Study:

  • To identify and characterize novel phage-encoded depolymerases targeting *Vibrio* pathogens.
  • To evaluate the antibiofilm activity and synergistic potential of a novel depolymerase (Dep193) in combination with a phage (VnaP).
  • To explore the therapeutic potential of phage depolymerases for controlling *Vibrio* infections in aquaculture.

Main Methods:

  • Bioinformatic analysis of *Vibrio* phages to identify putative depolymerase genes.
  • Isolation and characterization of a depolymerase-encoding phage (VnaP) and its associated depolymerase (Dep193).
  • Genome sequencing, heterologous expression, enzymatic activity assays, antibiofilm assays, and synergistic efficacy studies.

Main Results:

  • A high prevalence of putative depolymerase genes was found in *Vibrio* phages.
  • A novel polysaccharide depolymerase, Dep193, was identified and characterized, showing efficient degradation of *Vibrio* surface polysaccharides and potent antibiofilm activity.
  • The combination of Dep193 and phage VnaP demonstrated synergistic activity, significantly enhancing bacterial clearance and delaying resistance emergence in multiple *Vibrio* species.

Conclusions:

  • Dep193 is the first biochemically validated *Vibrio* phage depolymerase, expanding the known diversity of these enzymes.
  • The Dep193-VnaP combination represents a potent and synergistic therapeutic strategy for controlling *Vibrio* infections in aquaculture.
  • This study highlights the potential of phage-derived depolymerases as effective alternatives to antibiotics in combating resistant bacterial pathogens.