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An aryl-hydrocarbon receptor-based drug-screening strategy for hepatocellular carcinoma based on dynamic fluorescence
Yihong Sun1, Junyan Li1, Weiyao Sun1
1Shandong Provincial Key Laboratory of Tumor Imaging Equipment Development and Diagnosis & Treatment Integration Technology, School of Chemistry and Chemical Engineering, College of Medicine, Linyi University, Linyi 276000, P.R. China. shushzhang@126.com.
Abstract:
The development of fluorescence imaging-based drug-screening methods is crucial. We devised an AhR-specific platform AhRPH (LOD = 8.7 ng mL-1), which consists of vitamin-A functionalized liposomes targeting HSCs and encapsulating AhR-specific probes (ArP). It distinguished drug efficacy: sorafenib/lenvatinib boosted AhR (resistance), and regorafenib suppressed it. This method is specific/sensitive but has limited in vivo depth.
