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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Updated: Jan 14, 2026

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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MicroRNA-CRISPR biosensors for cancer diagnostics.

Alaa H Habib1, Ziaullah Mirza Sain2, Misbahuddin Rafeeq3

  • 1Department of Physiology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|January 12, 2026
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas systems offer sensitive detection of circulating microRNAs (miRNAs) for diagnosing cancer and cardiovascular diseases. These advanced biosensors show promise for clinical applications, overcoming challenges with current technologies.

Keywords:
CRISPR-CasCas12aCas13abiosensorscancer biomarkersclinical chemistryelectrochemical sensingliquid biopsymicroRNA

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Biomarker Discovery

Background:

  • Circulating microRNAs (miRNAs) are valuable biomarkers for cancer and cardiovascular diseases.
  • Low abundance, sequence homology, and matrix effects challenge miRNA detection in biofluids.
  • Existing analytical methods require improvement in sensitivity and robustness.

Purpose of the Study:

  • To review the latest advances in CRISPR-Cas based microRNA (miRNA) biosensors.
  • To evaluate the analytical performance and clinical translational potential of these biosensors.
  • To compare CRISPR-based workflows with conventional methods like RT-qPCR and digital PCR.

Main Methods:

  • Utilized CRISPR-Cas systems (Cas12a, Cas12b, Cas13a, Cas9) for programmable nucleic acid recognition.
  • Employed various signal amplification and readout strategies including fluorescence, electrochemical, and colorimetric methods.
  • Critically evaluated analytical performance metrics such as detection limits and specificity.

Main Results:

  • CRISPR-based miRNA biosensors achieve femtomolar to attomolar detection limits.
  • Demonstrated high specificity and mismatch discrimination capabilities.
  • Highlighted versatility across different biofluid samples (plasma, serum, saliva, whole blood, extracellular vesicles).

Conclusions:

  • CRISPR-Cas technology presents a highly sensitive and robust platform for miRNA detection.
  • These biosensors hold significant potential for minimally invasive clinical diagnostics.
  • Standardization, quality control, and clinical validation are crucial for widespread adoption in clinical laboratories.