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Related Experiment Video

Updated: Jan 14, 2026

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
06:35

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Published on: September 12, 2018

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Human Brain Vasculature-on-a-Chip Model Constructed With Microvessels Isolated From Cryopreserved Postmortem Human

Brian J O'Grady1,2, A Scott McCall3, Samuel Cullison4

  • 1Department of Ophthalmology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Advanced Healthcare Materials
|January 13, 2026
PubMed
Summary

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Researchers developed a novel human brain vasculature-on-a-chip model using microvessels from human cortical tissue. This biomimetic system accurately replicates brain vasculature structure and function for advanced research applications.

Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Vascular Biology

Background:

  • Brain vasculature is crucial for homeostasis but challenging to model accurately.
  • Existing models often lack anatomical relevance and in vivo functionality.
  • Need for better human brain vasculature models for drug screening and disease studies.

Purpose of the Study:

  • To develop a functional human brain vasculature-on-a-chip model.
  • To utilize microvessels from human cortical tissue for improved model fidelity.
  • To create a platform for drug screening and disease modeling.

Main Methods:

  • Isolated microvessels from cryopreserved human cortical tissue.
  • Cultured microvessels in a biomimetic gelatin-based hydrogel.
Keywords:
blood‐brain barrierin vitro modelneurovascular unitvasculature

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Related Experiment Videos

Last Updated: Jan 14, 2026

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Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
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  • Integrated the hydrogel construct into a microfluidic device.

    • Main Results:

    • Achieved anatomically relevant arteriole and capillary architectures within hydrogels.
    • Demonstrated lumen perfusion via anastomosis to hydrogel edges.
    • Confirmed intact blood-brain barrier function in capillaries via restricted dextran diffusion.

    Conclusions:

    • The developed human brain vasculature-on-a-chip is a functional and anatomically relevant model.
    • This platform shows promise for biotechnology applications, including drug screening.
    • Represents a significant advancement in modeling the complex brain vasculature.