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Acupotomy Activates PI3K/Akt Signaling Pathway Mediated by FGF7,10/KDR Axis to Regulate Apoptosis.

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Acupotomy treatment for cervical spondylosis (CS) effectively regulates apoptosis via the FGF7,10/KDR signaling axis, activating the PI3K/Akt pathway. This mechanism clarifies how acupotomy repairs muscles and treats bone disorders in CS.

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Area of Science:

  • Orthopedics
  • Molecular Biology
  • Traditional Chinese Medicine

Background:

  • Cervical spondylosis (CS) is a common condition with unclear treatment mechanisms.
  • Acupotomy is an effective clinical treatment for CS, often described as "regulating muscles to treat bone disorders."
  • The precise molecular mechanisms underlying acupotomy's efficacy in CS remain largely unknown.

Purpose of the Study:

  • To elucidate the molecular mechanism of acupotomy in treating cervical spondylosis (CS).
  • To investigate the role of cervical myocyte apoptosis and the PI3K/Akt signaling pathway.
  • To explore the FGF7,10/KDR signaling axis in mediating acupotomy's effects on CS.

Main Methods:

  • Construction of a CS rat model verified by X-ray and electron microscopy.
  • Assessment of protein interactions (FGF7, FGF10, KDR) using immunofluorescence and COIP.
  • Evaluation of motor and sensory function, pathological changes, and molecular expression (qPCR, Western blot).

Main Results:

  • Acupotomy improved motor and sensory function and reduced cervical degeneration in CS rats.
  • The FGF7,10/KDR signaling axis and PI3K/Akt pathway were implicated in CS.
  • Acupotomy reversed altered protein expressions, with KDR knockdown diminishing treatment effects.

Conclusions:

  • Acupotomy activates the PI3K/Akt pathway through the FGF7,10/KDR signaling axis.
  • This activation regulates cervical myocyte apoptosis and muscle repair.
  • This pathway represents a key molecular mechanism for acupotomy in treating CS.