Related Experiment Video
Updated: Jan 14, 2026

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
Published on: August 7, 2021
Translating the green code: Ribo-Seq for photosynthetic eukaryotes in focus
Naohiro Kawamoto1, Shintaro Iwasaki1,2
1RNA Systems Biochemistry Laboratory, Pioneering Research Institute RIKEN, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan.
Abstract:
Because they are continually exposed to fluctuating environments, photosynthetic organisms, including land plants and algae, must adapt to a wide range of environmental conditions. For this purpose, translational control plays a pivotal role. The advent of ribosome profiling, or Ribo-Seq, has helped overcome the technical barriers faced by earlier methods, enabling comprehensive and quantitative analysis of protein synthesis. This review highlights recent advances in green lineage Ribo-Seq, covering technical innovations, diverse applications and analytical insights. These findings emphasize the power and versatility of this technique in exploring translational regulation in photosynthetic species.
Related Concept Videos
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Ribosomal RNA Synthesis
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Photosystem II
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...

