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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Riboswitches01:56

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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Photosystem II01:22

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The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
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Updated: Jan 14, 2026

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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Translating the green code: Ribo-Seq for photosynthetic eukaryotes in focus.

Naohiro Kawamoto1, Shintaro Iwasaki1,2

  • 1RNA Systems Biochemistry Laboratory, Pioneering Research Institute RIKEN, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan.

Journal of Biochemistry
|January 13, 2026
PubMed
Summary

Photosynthetic organisms adapt to changing environments through translational control. Ribosome profiling (Ribo-Seq) offers a powerful method for studying protein synthesis and translational regulation in plants and algae.

Keywords:
RNA-binding proteinRibo-Seqchloroplastsmitochondriaribosome profilingtranslation

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Area of Science:

  • Plant Science
  • Molecular Biology
  • Genetics

Background:

  • Photosynthetic organisms face environmental fluctuations, necessitating adaptive mechanisms.
  • Translational control is crucial for regulating gene expression in response to environmental cues.
  • Traditional methods for studying protein synthesis have limitations.

Purpose of the Study:

  • To review recent advancements in ribosome profiling (Ribo-Seq) for photosynthetic organisms.
  • To highlight technical innovations, applications, and analytical insights of Ribo-Seq.
  • To underscore the utility of Ribo-Seq in understanding translational regulation.

Main Methods:

  • Ribosome profiling (Ribo-Seq) enables quantitative analysis of protein synthesis.
  • Review of technical innovations in Ribo-Seq methodology.
  • Examination of diverse applications of Ribo-Seq in green lineage species.

Main Results:

  • Ribo-Seq overcomes technical barriers of previous methods.
  • Provides comprehensive and quantitative data on protein synthesis.
  • Recent advances have expanded the scope and accuracy of Ribo-Seq.

Conclusions:

  • Ribo-Seq is a powerful and versatile technique for studying translational control.
  • Advances in Ribo-Seq offer new insights into gene expression regulation in plants and algae.
  • This technique is essential for exploring translational regulation in photosynthetic species.