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Related Experiment Video

Updated: Jan 14, 2026

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
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Taking "Multiple Shots on Goal" with an Armed Invariant NK Cell Approach.

Steven M Albelda1

  • 1Division of Pulmonary and Critical Care Medicine, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.

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|January 13, 2026
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A new therapy, MiNK-215, targets fibroblast activation protein on solid tumors. This novel chimeric antigen receptor invariant NK T-cell therapy enhances antitumor immunity and overcomes immunotherapy resistance in preclinical models.

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Area of Science:

  • Immunotherapy
  • Oncology
  • Cell Therapy

Background:

  • Adoptive T-cell therapies demonstrate limited effectiveness against solid tumors, necessitating innovative treatment strategies.
  • Fibroblast activation protein (FAP)-positive cancer-associated fibroblasts (CAFs) are key components of the tumor microenvironment that impede anti-tumor immunity.

Purpose of the Study:

  • To introduce MiNK-215, a novel allogeneic chimeric antigen receptor (CAR) invariant NK T-cell (iNKT) therapy designed to target FAP and enhance anti-tumor responses.
  • To evaluate the efficacy of MiNK-215 in remodeling the tumor microenvironment and overcoming resistance to immunotherapy.

Main Methods:

  • Development of MiNK-215, an allogeneic iNKT cell therapy engineered with a FAP-targeting CAR and IL-15 secretion.
  • Assessment of MiNK-215's ability to deplete FAP-positive CAFs and activate immune cells in mouse lung tumor models and human organoid systems.

Main Results:

  • MiNK-215 effectively remodeled the tumor microenvironment by depleting FAP-positive CAFs.
  • The therapy activated multiple immune cell types, leading to enhanced anti-tumor immunity.
  • MiNK-215 promoted durable, antigen-specific T-cell responses and overcame immunotherapy resistance in preclinical models.
  • No off-target toxicity was observed.

Conclusions:

  • MiNK-215 represents a promising novel cell therapy for solid tumors by targeting FAP-positive CAFs and enhancing anti-tumor immunity.
  • This approach demonstrates potential for overcoming immunotherapy resistance and offers a new avenue for treating solid tumors.