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Protocol for the injection of adenoviruses into brown adipose tissue in mice
Eva Prida1, José Adrián Vázquez-Durán2, Diego Muñoz-Moreno3
1Translational Endocrinology Group, Endocrinology Section, Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS)/Complexo Hospitalario Universitario de Santiago (SERGAS), Travesía da Choupana s/n, 15706 Santiago de Compostela, Galicia, Spain; CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Av Monforte de Lemos 3-5, 28029 Madrid, Spain.
Abstract:
Brown adipose tissue (BAT) represents a specialized form of adipose tissue that has attracted much attention in the search for therapeutic interventions to treat obesity and its related metabolic disorders. Here, we present a protocol to modulate BAT metabolism in vivo in mice via the direct administration of adenovirus (Ad). We also describe steps for the delivery of postoperative care, including the administration of analgesics and the application of antiseptics to the surgical site to prevent potential infections. For complete details on the use and execution of this protocol, please refer to Al-Massadi et al.1.
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