Supported Crystal/Amorphous Hybrid-Constructed Sensing Array: Exploiting Switchable Enzyme Activity for High
Miaomiao Song1, Weiguang Xiong2, Longzhao Zhang1
1School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, PR China.
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Nanozymes show great potential in environmental monitoring, yet their catalytic performance and recyclability remain challenging. This study developed a supported nanozyme system by pyrolyzing precursors to synthesize ultrafine CuO nanoclusters anchored on hollow sea-urchin-shaped SiO2 (CuO@SiO2). The material exhibits switchable peroxidase- and laccase-like activities, strong substrate affinity, and excellent recyclability, attributable to its unique crystal/amorphous hybrid structure. Leveraging this dual-enzyme activity, we constructed a dual-channel sensor array based on the "turn-on" laccase-like and "turn-off" peroxidase-like responses, enabling highly selective discrimination among dihydroxybenzene isomers at concentrations as low as 5 μM. The array successfully identified single isomers, mixed samples, and nine unknown analytes with 100% accuracy. This work provides new insights into supported nanozyme design and demonstrates a multidimensional signal extraction strategy to advance nanozyme sensing technology.
