Bioprinting collagenase-responsive hydrogel for controlled release of cowpea mosaic virus immunotherapy
Zhongchao Zhao1,2,3,4, Yi Xiang1, Zhuohong Wu1
1Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, United States of America.
Abstract:
In this work, we developed a collagenase-responsive hydrogel system to covalently load cancer immunotherapy candidate cowpea mosaic virus (CPMV) using 3D digital light processing bioprinting technology. CPMV was functionalized with norbornene groups (CPMV-NB), which was then bioprinted into hydrogels with 8-arm polyethylene glycol norbornene and a collagenase-cleavable peptide via photoinduced thiol-ene click chemistry. This strategy enabled stable retention of CPMV-NB within the hydrogels and achieved controlled release of CPMV-NB triggered by collagenase. Furthermore, released CPMV-NB retained its immunogenicity to stimulate immune cells.
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