Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Small GTPases - Ras and Rho

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Early life Oxytocin treatment Attenuates Seizure Susceptibility in Male, but not Female, <i>Fmr1</i> -KO Mice.

bioRxiv : the preprint server for biology·2026

Same author

A depression-like phenotype is associated with discrete defects in the primary hippocampal circuit.

Translational psychiatry·2026

Same author

Lasting effects of early-life oxytocin treatment on LTP and episodic memory in a mouse model of Fragile X syndrome.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026

Same author

A concise mathematical description of signal transformations across the hippocampal apical CA3 to CA1 dendritic response.

Frontiers in neural circuits·2026

Same author

Hippocampal estrogen levels, receptor types, and epigenetics contribute to sex-specific memory vulnerabilities to concurrent acute stresses.

Neuron·2026

Same author

LTP: A Personal Journey and Beyond.

Hippocampus·2026

Same journal

Vestibular function drives gaze stability in locomoting macaques.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same journal

Region- and layer-specific glutamatergic synapse development in the nascent cortical hierarchy.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same journal

Endogenous peptide derived from c-Cbl-associated protein counteracts its inhibitory effect on enteric neural crest cell colonization in Hirschsprung disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same journal

Drowsiness alters the neural dynamics but not the core computations of multisensory integration.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same journal

A Matter of Parameters: Tailored Transcranial Focused Ultrasound Enhances Cortico-Thalamo-Cortical Circuit Resonance.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same journal

Proactive visual and motor prioritization differentially scale with cue reliability.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

Localization and Functional Characterization of MDGA1 in Mouse Hippocampus.

Matthew A Sandoval^1,2, Michael A Bemben³, Gerardo Leana-Sandoval^1,2

¹Department of Anatomy & Neurobiology, University of California at Irvine, Irvine, California 92617.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience

|January 13, 2026

Summary

This summary is machine-generated.

The MAM domain-containing glycosylphosphatidylinositol anchor protein 1 (MDGA1) is not enriched at synapses and does not regulate synaptic transmission in young mice. This finding clarifies MDGA1

Keywords:

MDGA1 cell adhesion molecule excitatory synaptic transmission hippocampal field CA1 inhibitory synaptic transmission

More Related Videos

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Published on: March 31, 2015

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

Published on: May 12, 2015

Related Experiment Videos

Last Updated: Jun 21, 2026

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Published on: March 31, 2015

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

Published on: May 12, 2015

Area of Science:

Neuroscience
Molecular Biology
Synaptic Plasticity

Background:

Synaptic organizer molecules are crucial for neuron communication, synapse formation, and plasticity.
MDGA1, a GPI-anchored protein, has been proposed to repress synapse formation and is linked to psychiatric disorders.
Previous studies on MDGA1's in vivo localization and function have yielded conflicting results.

Purpose of the Study:

To precisely define the localization and function of MDGA1 in the mouse brain.
To investigate MDGA1's role in regulating synaptic transmission in the hippocampus.

Main Methods:

Generation of epitope-tagged MDGA1 knock-in mice (male and female).
Analysis of MDGA1 expression patterns in the forebrain during postnatal development.
Slice electrophysiology to assess the impact of acute MDGA1 deletion on synaptic transmission in hippocampal CA1.

Main Results:

MDGA1 expression peaks in the forebrain during early postnatal weeks.
In hippocampal CA1, MDGA1 localizes to dendrites but not specifically to excitatory or inhibitory synapses.
Acute deletion of MDGA1 did not affect excitatory or inhibitory synaptic transmission.

Conclusions:

MDGA1 transiently inhabits hippocampal synapses in young mice but is not a strong regulator of synaptic transmission.
These findings challenge the proposed role of MDGA1 as a direct synaptic organizer molecule.
Further research is needed to fully elucidate MDGA1's function in the nervous system.