Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Stability01:53

RNA Stability

35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.5K
Experimental RNAi02:15

Experimental RNAi

7.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.3K
RNA Interference01:23

RNA Interference

27.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endoscopic Coagulation with Clipping for Colonic Diverticular Bleeding: A Practical Hemostatic Strategy.

Endoscopy international open·2026
Same author

Conversion surgery after response to zolbetuximab-based chemotherapy in Claudin 18.2-positive gastric cancer: a case report.

Journal of surgical case reports·2026
Same author

Rediscovery of "Gloeocystis-Halbflechte" after 84 years revealed an independent lineage of ascomycetes harboured in gelatinous algal biofilms.

Fungal biology·2026
Same author

Preoperative and Pathological Discrepancies in Colorectal Cancer: A Single-Institution Retrospective Analysis Based on Mismatch Repair/<i>BRAF</i> V600E Status.

Journal of the anus, rectum and colon·2026
Same author

Robotic-assisted transverse colectomy for transverse colon cancer in a centenarian: a case report.

Journal of surgical case reports·2026
Same author

Dual regulation of appressorium development in the maize pathogen <i>Bipolaris maydis</i>: BmOPY2-mediated hydrophobic surface recognition and pectin recognition.

Mycoscience·2026

Related Experiment Video

Updated: Jan 15, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

10.6K

Improved Messenger RNA Stability and Biocompatibility Through Self-Gelatinizable Nucleic Acids.

Takumi Tanifuji1, Kosuke Kusamori2, Chihiro Tanaka1

  • 1Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Katsushika, Tokyo, Japan.

Biotechnology and Bioengineering
|January 14, 2026
PubMed
Summary

Researchers developed a novel DNA hydrogel to stabilize messenger RNA (mRNA) therapeutics. This biocompatible platform enhances mRNA stability and reduces immunogenicity, offering a promising alternative to lipid nanoparticles for future therapies.

Keywords:
DNA hydrogelbiocompatibilityimmunogenicitymessenger RNAself‐assemblysustained release

More Related Videos

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells
10:02

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

2.6K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

5.0K

Related Experiment Videos

Last Updated: Jan 15, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

10.6K
Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells
10:02

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

2.6K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

5.0K

Area of Science:

  • Biotechnology
  • Materials Science
  • Molecular Biology

Background:

  • Messenger RNA (mRNA) therapeutics show promise but face challenges with in vivo instability and storage.
  • Current lipid nanoparticle (LNP) delivery systems, while successful, can elicit immunogenic responses.
  • Safer and more stable delivery platforms are needed for advanced mRNA therapies.

Purpose of the Study:

  • To develop a novel, biocompatible delivery system for mRNA therapeutics.
  • To address the limitations of mRNA instability and LNP immunogenicity.
  • To evaluate the efficacy and safety of mRNA-loaded DNA hydrogels.

Main Methods:

  • Designed DNA units were used to create self-assembling DNA hydrogels.
  • Messenger RNA was loaded into the DNA hydrogel framework.
  • In vitro assays were performed to assess protein expression, mRNA stability, and immunogenicity in relevant cell types (myofibroblasts and antigen-presenting cells).

Main Results:

  • The DNA hydrogel demonstrated sustained mRNA-mediated protein expression in myofibroblasts.
  • The hydrogel significantly enhanced resistance of mRNA to nuclease and storage degradation.
  • Negligible proinflammatory cytokine production and cytotoxicity were observed in antigen-presenting cells.

Conclusions:

  • mRNA-loaded DNA hydrogels represent a promising, biocompatible platform for next-generation mRNA therapeutics.
  • This technology offers enhanced mRNA stability and reduced immunogenicity compared to existing methods.
  • DNA hydrogels provide a viable alternative for developing safer and more effective mRNA-based treatments.