Modeling spectroradiometric measurements of oral mucosal tissue autofluorescence

Spectroradiometric fluorescence measurements were collected from the dorsal tongue and inner lip of healthy volunteers. These sites were chosen to represent the distinct spectral features that differentiate keratinized from non-keratinized oral tissues, as documented in previous studies. A computational model was then applied to estimate the relative contributions of key fluorophores and to quantify the influence of blood absorption on the observed fluorescence spectra. The resulting dataset and model, both freely available, serve as reference standards for healthy oral tissue and support the development of quantitative, non-invasive imaging systems for consistent and reproducible assessment of oral mucosal health.