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Related Concept Videos

Computed Tomography01:10

Computed Tomography

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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Transmission Electron Microscopy01:15

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
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Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
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Updated: Jan 15, 2026

Doppler Optical Coherence Tomography of Retinal Circulation
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qtOCT: quantitative transmission optical coherence tomography.

Wojciech Krauze1, Martyna Mazur1, Arkadiusz Kuś1

  • 1Warsaw University of Technology, Institute of Micromechanics and Photonics, Boboli 8 Street, 02-525, Warsaw, Poland.

Biomedical Optics Express
|January 14, 2026
PubMed
Summary
This summary is machine-generated.

A new quantitative transmission optical coherence tomography (OCT) method, qtOCT, directly retrieves phase information from biological specimens. This technique offers a faster and more rigorous alternative to existing qualitative methods for advanced imaging.

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Area of Science:

  • Biomedical Optics
  • Quantitative Phase Imaging
  • Optical Coherence Tomography

Background:

  • Transmission optical coherence tomography (OCT) analyzes biological samples via forward-scattered light.
  • Current transmission OCT is primarily qualitative, lacking direct quantitative phase retrieval capabilities.
  • Existing methods for quantitative phase imaging can be complex and time-consuming.

Purpose of the Study:

  • To introduce qtOCT, a novel quantitative transmission OCT method.
  • To enable direct, fast, and rigorous retrieval of 2D integrated phase information.
  • To enhance transmission OCT for analyzing both weak and multiple-scattering biological samples.

Main Methods:

  • Utilized transmission full-field swept-source OCT measurements.
  • Implemented coherence gating for precise signal isolation.
  • Enabled user-defined temporal measurement range selection for adaptability.

Main Results:

  • Demonstrated high consistency between qtOCT and digital holographic microscopy in the weak-scattering regime.
  • Showcased the superiority of qtOCT in analyzing multiple-scattering biological samples.
  • Successfully retrieved 2D integrated phase information directly and efficiently.

Conclusions:

  • qtOCT significantly enhances the capabilities of transmission OCT.
  • This method provides a viable and advantageous alternative to existing quantitative phase imaging techniques.
  • qtOCT offers a direct, fast, and rigorous approach for phase retrieval in OCT.